Single Doses of Acrylamide Reduce Retrograde Transport Velocity

Matthew S. Miller, Peter S. Spencer

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Abstract: Single doses of acrylamide (0–1.3 mmol/kg) produced a dose‐dependent decrease in the transport of 125I‐tetanus toxin to the perikarya of sensory neurons in dorsal root ganglia and motor neurons in ventral spinal cord. Acrylamide was a more potent inhibitor of retrograde transport in sensory axons than in motor axons. Substantially greater doses of N,N′‐methylene‐bis‐acryl‐amide, a reportedly non‐neurotoxic analog of acrylamide, were required to alter the axonal transport of 125I‐tetanus toxin. Velocity of retrograde transport was assessed by determining the position of the leading edge of transported125I‐tetanus toxin at times following single doses of acrylamide. Acrylamide reduced the velocity of 125I‐tetanus toxin transport in a dose‐dependent manner by up to 75%. No change in neuronal uptake of 125I‐tet‐anus toxin was detected. It is concluded that single doses of acrylamide produce profound alterations in retrograde transport which precede the appearance of structural changes in affected nerve fibers.

Original languageEnglish (US)
Pages (from-to)1401-1408
Number of pages8
JournalJournal of neurochemistry
Issue number5
StatePublished - Nov 1984
Externally publishedYes


  • Acrylamide
  • Axoplasmic transport
  • Neurotoxicity

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience


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