Single Doses of Acrylamide Reduce Retrograde Transport Velocity

Matthew S. Miller, Peter S. Spencer

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Abstract: Single doses of acrylamide (0–1.3 mmol/kg) produced a dose‐dependent decrease in the transport of 125I‐tetanus toxin to the perikarya of sensory neurons in dorsal root ganglia and motor neurons in ventral spinal cord. Acrylamide was a more potent inhibitor of retrograde transport in sensory axons than in motor axons. Substantially greater doses of N,N′‐methylene‐bis‐acryl‐amide, a reportedly non‐neurotoxic analog of acrylamide, were required to alter the axonal transport of 125I‐tetanus toxin. Velocity of retrograde transport was assessed by determining the position of the leading edge of transported125I‐tetanus toxin at times following single doses of acrylamide. Acrylamide reduced the velocity of 125I‐tetanus toxin transport in a dose‐dependent manner by up to 75%. No change in neuronal uptake of 125I‐tet‐anus toxin was detected. It is concluded that single doses of acrylamide produce profound alterations in retrograde transport which precede the appearance of structural changes in affected nerve fibers.

Original languageEnglish (US)
Pages (from-to)1401-1408
Number of pages8
JournalJournal of Neurochemistry
Volume43
Issue number5
DOIs
StatePublished - Nov 1984

Keywords

  • Acrylamide
  • Axoplasmic transport
  • Neurotoxicity

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Single Doses of Acrylamide Reduce Retrograde Transport Velocity'. Together they form a unique fingerprint.

  • Cite this