Single-cell XIST expression in human preimplantation embryos and newly reprogrammed female induced pluripotent stem cells

Sharon F. Briggs, Antonia A. Dominguez, Shawn Chavez, Renee A. Reijo Pera

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The process of X chromosome inactivation (XCI) during reprogramming to produce human induced pluripotent stem cells (iPSCs), as well as during the extensive programming that occurs in human preimplantation development, is not well-understood. Indeed, studies of XCI during reprogramming to iPSCs report cells with two active X chromosomes and/or cells with one inactive X chromosome. Here, we examine expression of the long noncoding RNA, XIST, in single cells of human embryos through the oocyte-to-embryo transition and in new mRNA reprogrammed iPSCs. We show that XIST is first expressed beginning at the 4-cell stage, coincident with the onset of embryonic genome activation in an asynchronous manner. Additionally, we report that mRNA reprogramming produces iPSCs that initially express XIST transcript; however, expression is rapidly lost with culture. Loss of XIST and H3K27me3 enrichment at the inactive X chromosome at late passage results in X chromosome expression changes. Our data may contribute to applications in disease modeling and potential translational applications of female stem cells.

Original languageEnglish (US)
Pages (from-to)1771-1781
Number of pages11
JournalStem Cells
Volume33
Issue number6
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Fingerprint

Induced Pluripotent Stem Cells
X Chromosome
Blastocyst
X Chromosome Inactivation
Embryonic Structures
Long Noncoding RNA
Messenger RNA
Human Development
Oocytes
Stem Cells
Genome

Keywords

  • Human
  • Human induced pluripotent stem cells
  • Preimplantation embryo development
  • X chromosome inactivation
  • XIST RNA

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Single-cell XIST expression in human preimplantation embryos and newly reprogrammed female induced pluripotent stem cells. / Briggs, Sharon F.; Dominguez, Antonia A.; Chavez, Shawn; Reijo Pera, Renee A.

In: Stem Cells, Vol. 33, No. 6, 01.06.2015, p. 1771-1781.

Research output: Contribution to journalArticle

Briggs, Sharon F. ; Dominguez, Antonia A. ; Chavez, Shawn ; Reijo Pera, Renee A. / Single-cell XIST expression in human preimplantation embryos and newly reprogrammed female induced pluripotent stem cells. In: Stem Cells. 2015 ; Vol. 33, No. 6. pp. 1771-1781.
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