Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development

Thorsten Boroviak, Giuliano G. Stirparo, Sabine Dietmann, Irene Hernando-Herraez, Hisham Mohammed, Wolf Reik, Austin Smith, Erika Sasaki, Jennifer Nichols, Paul Bertone

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The mouse embryo is the canonical model for mammalian preimplantation development. Recent advances in single cell profiling allow detailed analysis of embryogenesis in other eutherian species, including human, to distinguish conserved from divergent regulatory programs and signalling pathways in the rodent paradigm. Here, we identify and compare transcriptional features of human, marmoset and mouse embryos by single cell RNA-seq. Zygotic genome activation correlates with the presence of polycomb repressive complexes in all three species, while ribosome biogenesis emerges as a predominant attribute in primate embryos, supporting prolonged translation of maternally deposited RNAs. We find that transposable element expression signatures are species, stage and lineage specific. The pluripotency network in the primate epiblast lacks certain regulators that are operative in mouse, but encompasses WNT components and genes associated with trophoblast specification. Sequential activation of GATA6, SOX17 and GATA4 markers of primitive endoderm identity is conserved in primates. Unexpectedly, OTX2 is also associated with primitive endoderm specification in human and non-human primate blastocysts. Our cross-species analysis demarcates both conserved and primate-specific features of preimplantation development, and underscores the molecular adaptability of early mammalian embryogenesis.

Original languageEnglish (US)
Article numberdev167833
JournalDevelopment (Cambridge)
Volume145
Issue number21
DOIs
StatePublished - 2018
Externally publishedYes

Keywords

  • Blastocyst
  • Embryo
  • Human
  • Inner cell mass
  • Pluripotency
  • Primate

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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