Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas

Yue J. Wang, Maria L. Golson, Jonathan Schug, Daniel Traum, Chengyang Liu, Kumar Vivek, Craig Dorrell, Ali Naji, Alvin C. Powers, Kyong Mi Chang, Markus Grompe, Klaus H. Kaestner

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High-dimensional analysis of our marker protein expression revealed three major clusters of beta cells within individuals. Proliferating beta cells are confined to two of the clusters.

Original languageEnglish (US)
Pages (from-to)616-626
Number of pages11
JournalCell Metabolism
Volume24
Issue number4
DOIs
StatePublished - Oct 11 2016

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Wang, Y. J., Golson, M. L., Schug, J., Traum, D., Liu, C., Vivek, K., Dorrell, C., Naji, A., Powers, A. C., Chang, K. M., Grompe, M., & Kaestner, K. H. (2016). Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas. Cell Metabolism, 24(4), 616-626. https://doi.org/10.1016/j.cmet.2016.09.007