Single cell lineage tracing reveals a role for TgfβR2 in intestinal stem cell dynamics and differentiation

Jared Fischer, Peter P. Calabrese, Ashleigh J. Miller, Nina M. Munoz, William M. Grady, Darryl Shibata, Robert (Mike) Liskay

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Intestinal stem cells (ISCs) are maintained by a niche mechanism, in which multiple ISCs undergo differential fates where a single ISC clone ultimately occupies the niche. Importantly, mutations continually accumulate within ISCs creating a potential competitive niche environment. Here we use single cell lineage tracing following stochastic transforming growth factor β receptor 2 (TgfβR2) mutation to show cell autonomous effects of TgfβR2 loss on ISC clonal dynamics and differentiation. Specifically, TgfβR2 mutation in ISCs increased clone survival while lengthening times to monoclonality, suggesting that Tgfβ signaling controls both ISC clone extinction and expansion, independent of proliferation. In addition, TgfβR2 loss in vivo reduced crypt fission, irradiation-induced crypt regeneration, and differentiation toward Paneth cells. Finally, altered Tgfβ signaling in cultured mouse and human enteroids supports further the in vivo data and reveals a critical role for Tgfβ signaling in generating precursor secretory cells. Overall, our data reveal a key role for Tgfβ signaling in regulating ISCs clonal dynamics and differentiation, with implications for cancer, tissue regeneration, and inflammation.

Original languageEnglish (US)
Pages (from-to)12192-12197
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number43
DOIs
Publication statusPublished - Oct 25 2016

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Keywords

  • Intestinal stem cell
  • Lgr5
  • Paneth cell
  • Tgfβ
  • TgfβR2

ASJC Scopus subject areas

  • General

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