Simultaneous visualization of two cellular mRNA species in individual neurons by use of a new double in Situ hybridization method

Daniel Marks, Jeffrey N. Wiemann, Kimberly A. Burton, Karin L. Lent, Donald K. Clifton, Robert A. Steiner

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We present a new method for the simultaneous detection of two mRNA species within individual neurons. The technique involves the use of radio-labeled and digoxigenin-labeled cRNA probes, the application of which confers a high specificity and sensitivity to the in situ hybridization analysis. We demonstrate the use of this method by illustrating the coexpression of preprogonadotropin-releasing hormone (GnRH) mRNA and preprogalanin mRNA in neurons in the rat forebrain and report a distinct sexual dimorphism in galanin gene expression by GnRH neurons. Coupling this technology with semi-quantitative analysis of the mRNA species hybridized with the isotopically labeled mRNA would permit studies of gene regulation in individual cells among the heterogeneous populations of the brain.

Original languageEnglish (US)
Pages (from-to)395-405
Number of pages11
JournalMolecular and Cellular Neuroscience
Volume3
Issue number5
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

In Situ Hybridization
Neurons
Messenger RNA
Gonadotropin-Releasing Hormone
Galanin
Digoxigenin
Complementary RNA
Prosencephalon
Radio
Sex Characteristics
Hormones
Technology
Gene Expression
Sensitivity and Specificity
Brain
Population
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Simultaneous visualization of two cellular mRNA species in individual neurons by use of a new double in Situ hybridization method. / Marks, Daniel; Wiemann, Jeffrey N.; Burton, Kimberly A.; Lent, Karin L.; Clifton, Donald K.; Steiner, Robert A.

In: Molecular and Cellular Neuroscience, Vol. 3, No. 5, 1992, p. 395-405.

Research output: Contribution to journalArticle

Marks, Daniel ; Wiemann, Jeffrey N. ; Burton, Kimberly A. ; Lent, Karin L. ; Clifton, Donald K. ; Steiner, Robert A. / Simultaneous visualization of two cellular mRNA species in individual neurons by use of a new double in Situ hybridization method. In: Molecular and Cellular Neuroscience. 1992 ; Vol. 3, No. 5. pp. 395-405.
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