Simultaneous recording of multiple cellular events by FRET

Alen Piljic, Carsten Schultz

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

The function of many sensors for measuring intracellular events is based on Förster resonance energy transfer (FRET). Here we demonstrate for the first time the use of multiple ratiometric FRET sensors in parallel through spatial and spectral resolution. We monitored three calcium-dependent signaling events by a cytosolic sensor for calcium/calmodulin-dependent protein kinase IIα, a membrane-bound sensor for protein kinase C, and a translocating FRET probe based on annexin A4. This multiparameter imaging approach gives insight into the precise timing of cellular events within one single cell, thereby providing a major advantage over single-parameter protocols. This type of imaging will likely be important for high content cell analysis and screening efforts in the future.

Original languageEnglish (US)
Pages (from-to)156-160
Number of pages5
JournalACS chemical biology
Volume3
Issue number3
DOIs
StatePublished - Mar 20 2008

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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