Simultaneous multicompartment intracellular Ca2+ measurements in the perfused heart using 19F NMR spectroscopy

Shigeki Yanagida, Cameron S. Luo, James A. Balschi, Gerald M. Pohost, Martin Pike

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Although Ca2+ transport regulation at subcellular organelles is of great interest, only limited methodology has been available for measuring organellar [Ca2+] levels. The present study employs the 19F NMR resonance frequency of 4F-BAPTA to measure free [Ca2+]. In 4F-BAPTA loaded perfused rabbit hearts, two 19F NMR resonances were clearly observed. The frequency of one was consistent with cytosolic [Ca2+] levels. Responses to agents that alter sarcoplasmic reticulum function identified the other resonance as originating from that organelle. The experiments demonstrate the utility of NMR shift indicator methodology in obtaining simultaneous multicompartment intracellular [Ca2+] measurements and in enabling organellar [Ca2+] measurements to be made from within intact living tissue.

Original languageEnglish (US)
Pages (from-to)640-647
Number of pages8
JournalMagnetic Resonance in Medicine
Volume35
Issue number5
StatePublished - May 1996
Externally publishedYes

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Organelles
Magnetic Resonance Spectroscopy
Sarcoplasmic Reticulum
Rabbits
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid

Keywords

  • Calcium
  • Myocardium
  • NMR
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Simultaneous multicompartment intracellular Ca2+ measurements in the perfused heart using 19F NMR spectroscopy. / Yanagida, Shigeki; Luo, Cameron S.; Balschi, James A.; Pohost, Gerald M.; Pike, Martin.

In: Magnetic Resonance in Medicine, Vol. 35, No. 5, 05.1996, p. 640-647.

Research output: Contribution to journalArticle

Yanagida, Shigeki ; Luo, Cameron S. ; Balschi, James A. ; Pohost, Gerald M. ; Pike, Martin. / Simultaneous multicompartment intracellular Ca2+ measurements in the perfused heart using 19F NMR spectroscopy. In: Magnetic Resonance in Medicine. 1996 ; Vol. 35, No. 5. pp. 640-647.
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