Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies.

Ronald (Ron) Rosenfeld, D. Baldwin, L. A. Dollar, R. L. Hintz, J. M. Olefsky, A. Rubenstein

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A 53-y4-old male patient with insulin-resistant diabetes was found to have circulating inhibitors of both insulin and somatomedin-C binding. Serum obtained from the patient at the time of initial presentation inhibited 50% of both 125I-insulin and 125I-SM-C binding to IM-9 lymphocytes at dilutions of 1:150. Spontaneous improvement in the patient's diabetic state was associated with a simultaneous and equal decrease in the serum inhibitory titers for both radioligands. Scatchard analysis indicated that the observed serum-induced decrease in both insulin and SM-C binding was due to decreased receptor affinity, with no alteration in receptor number. The serum inhibitors of both insulin and SM-C binding were precipitated equally by Staph-A and also by 40% ammonium sulfate, suggesting they were immunoglobulins. The observation of naturally occurring autoantibodies against both the insulin and SM-C receptors suggests a structural homology between the two receptors.

Original languageEnglish (US)
Pages (from-to)979-982
Number of pages4
JournalDiabetes
Volume30
Issue number11
StatePublished - Nov 1981
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor I
Lymphocytes
Insulin
Antibodies
Serum
Ammonium Sulfate
Autoantibodies
Immunoglobulins
Observation

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Internal Medicine

Cite this

Rosenfeld, R. R., Baldwin, D., Dollar, L. A., Hintz, R. L., Olefsky, J. M., & Rubenstein, A. (1981). Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies. Diabetes, 30(11), 979-982.

Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies. / Rosenfeld, Ronald (Ron); Baldwin, D.; Dollar, L. A.; Hintz, R. L.; Olefsky, J. M.; Rubenstein, A.

In: Diabetes, Vol. 30, No. 11, 11.1981, p. 979-982.

Research output: Contribution to journalArticle

Rosenfeld, RR, Baldwin, D, Dollar, LA, Hintz, RL, Olefsky, JM & Rubenstein, A 1981, 'Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies.', Diabetes, vol. 30, no. 11, pp. 979-982.
Rosenfeld RR, Baldwin D, Dollar LA, Hintz RL, Olefsky JM, Rubenstein A. Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies. Diabetes. 1981 Nov;30(11):979-982.
Rosenfeld, Ronald (Ron) ; Baldwin, D. ; Dollar, L. A. ; Hintz, R. L. ; Olefsky, J. M. ; Rubenstein, A. / Simultaneous inhibition of insulin and somatomedin-C binding to cultured IM-9 lymphocytes by naturally occurring antireceptor antibodies. In: Diabetes. 1981 ; Vol. 30, No. 11. pp. 979-982.
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