Similar pattern of MCP-1 expression in spinal cords and eyes of Lewis rats with experimental autoimmune encephalomyelitis associated anterior uveitis

Grazyna Adamus, Michal Machnicki, Drake Amundson, Kirsten Adlard, Halina Offner

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Monocyte chemoattractant protein-1 (MCP-1) is a member of the CC chemokine family responsible for the recruitment of T cells that have been found during inflammation of the spinal cord in experimental autoimmune encephalomyelitis (EAE) in Lewis rats immunized with myelin basic protein (MBP). Lewis rats injected with MBP also developed anterior uveitis (AU), which coincided with the onset of EAE. In the present studies, we examined the expression and distribution of MCP-1 in the eye and spinal cord during disease and compared it to the expression of Th1 cell type cytokines. Initially, MCP-1 expression was detected at the preclinical phase in the iris/ciliary body and lumbar spinal cord and increased during the course of EAE/AU. Mononuclear infiltrating cells and endothelial cells and astrocytes of the CNS could be identified as a source of MCP-1 by in situ hybridization. Kinetics of expression of Th1 characteristic cytokines such as IL-2 and IFNQγ was in agreement with the expression of MCP-1 chemokine. Moreover, induction of the gene expression of MCP-1 seemed to occur earlier than that of MIP-2, and it correlated with increasing disease severity. MCP-1 seems to contribute to the initial recruitment of inflammatory cells into both the tissues of the eye and CNS over the course of disease.

Original languageEnglish (US)
Pages (from-to)531-538
Number of pages8
JournalJournal of Neuroscience Research
Volume50
Issue number4
DOIs
StatePublished - Nov 15 1997

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Anterior Uveitis
Autoimmune Experimental Encephalomyelitis
Chemokine CCL2
Spinal Cord
Myelin Basic Protein
Cytokines
Myelitis
CC Chemokines
Ciliary Body
Th1 Cells
Spinal Cord Diseases
Iris
Chemokines
Astrocytes
Interleukin-2
In Situ Hybridization
Endothelial Cells
T-Lymphocytes
Gene Expression

Keywords

  • Autoimmunity
  • Chemokine
  • Eye
  • Iritis
  • Myelin basic protein
  • T cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Similar pattern of MCP-1 expression in spinal cords and eyes of Lewis rats with experimental autoimmune encephalomyelitis associated anterior uveitis. / Adamus, Grazyna; Machnicki, Michal; Amundson, Drake; Adlard, Kirsten; Offner, Halina.

In: Journal of Neuroscience Research, Vol. 50, No. 4, 15.11.1997, p. 531-538.

Research output: Contribution to journalArticle

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