Simian retrovirus serogroup 5

Partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque

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17 Citations (Scopus)

Abstract

The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalVirus Genes
Volume21
Issue number3
DOIs
StatePublished - 2000

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Simian Retroviruses
Viral RNA
Macaca mulatta
Salivary Glands
Reverse Transcriptase Polymerase Chain Reaction
Simian Acquired Immunodeficiency Syndrome
Bone Marrow
Messenger RNA
Seminal Vesicles
Submandibular Gland
Macaca
Tissue Distribution
Northern Blotting
Hyperplasia
Sequence Analysis
Prostate
Skeletal Muscle
Spleen
Kidney
Infection

Keywords

  • Dot blot
  • Generalized lymphoid hyperplasia
  • Northern blot analyses
  • Partial sequence of D5/RHE/OR gag-prt product
  • Partial sequence of rhesus macaque cyclophilin product
  • Reverse transcriptase-polymerase chain reaction (RT-PCR)
  • Simian retrovirus (SRV) serogroup 5
  • Tissue distribution analyses of D5/RHE/OR RNA

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

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title = "Simian retrovirus serogroup 5: Partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque",
abstract = "The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.",
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author = "B. Li and Michael Axthelm and Curtis Machida",
year = "2000",
doi = "10.1023/A:1008104000920",
language = "English (US)",
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pages = "241--248",
journal = "Virus Genes",
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T2 - Partial gag-prt sequence and viral RNA distribution in an infected rhesus macaque

AU - Li, B.

AU - Axthelm, Michael

AU - Machida, Curtis

PY - 2000

Y1 - 2000

N2 - The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.

AB - The simian type D retroviruses (SRVs) are one of the causative agents of simian acquired immunodeficiency syndrome (SAIDS) in Asian macaques. In this report, we describe the infection of a rhesus macaque with the SRV serogroup 5 isolate, D5/RHE/OR. Using reverse transcriptase-polymerase chain reaction (RT-PCR) and dot blot hybridization analyses, we have determined the tissue distribution of D5/RHE/OR in this infected rhesus macaque, and have demonstrated viral mRNA in the majority of the surveyed tissues, including robust loads in the bone marrow, seminal vesicle, submaxillary salivary gland, prostate, and skeletal muscle. Microscopic examination of necropsy tissues revealed generalized lymphoid hyperplasia that was most severe in the salivary gland, bone marrow, kidney, and spleen. We also describe the first sequence analyses of portions of the D5/RHE/OR gag-prt region, obtained as a RT-PCR amplification product from infected rhesus macaque tissue, and report the first confirmation using Northern blot analyses that the SRV serogroups, including D5/RHE/OR, express similarly-sized genomic and subgenomic env mRNAs.

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KW - Reverse transcriptase-polymerase chain reaction (RT-PCR)

KW - Simian retrovirus (SRV) serogroup 5

KW - Tissue distribution analyses of D5/RHE/OR RNA

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