Simian Immunodeficiency Virus SIVmac239Δnef vaccination elicits different Tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques

Benjamin J. Burwitz, Zachary Ende, Benjamin Sudolcan, Matthew R. Reynolds, Justin M. Greene, Benjamin N. Bimber, Jorge R. Almeida, Monica Kurniawan, Vanessa Venturi, Emma Gostick, Roger W. Wiseman, Daniel C. Douek, David A. Price, David H. O'Connor

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Different human immunodeficiency virus (HIV)/simian immunodeficiency virus (SIV) vaccine vectors expressing the same viral antigens can elicit disparate T-cell responses. Within this spectrum, replicating variable vaccines, like SIVmac239Δnef, appear to generate particularly efficacious CD8+ T-cell responses. Here, we sequenced T-cell receptor β-chain (TRB) gene rearrangements from immunodominant Mamu-A*01-restricted Tat 28-35SL8-specific CD8+ T-cell populations together with the corresponding viral epitope in four rhesus macaques during acute SIVmac239Δnef infection. Ultradeep pyrosequencing showed that viral variants arose with identical kinetics in SIVmac239Δnef and pathogenic SIVmac239 infection. Furthermore, distinct Tat28-35SL8-specific T-cell receptor (TCR) repertoires were elicited by SIVmac239Δnef compared to those observed following a DNA/Ad5 prime-boost regimen, likely reflecting differences in antigen sequence stability.

Original languageEnglish (US)
Pages (from-to)3683-3689
Number of pages7
JournalJournal of virology
Volume85
Issue number7
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Burwitz, B. J., Ende, Z., Sudolcan, B., Reynolds, M. R., Greene, J. M., Bimber, B. N., Almeida, J. R., Kurniawan, M., Venturi, V., Gostick, E., Wiseman, R. W., Douek, D. C., Price, D. A., & O'Connor, D. H. (2011). Simian Immunodeficiency Virus SIVmac239Δnef vaccination elicits different Tat28-35SL8-specific CD8+ T-cell clonotypes compared to a DNA prime/adenovirus type 5 boost regimen in rhesus macaques. Journal of virology, 85(7), 3683-3689. https://doi.org/10.1128/JVI.02112-10