Silicalite zeolite: A novel matrix for purification and estimation of a hydrophobic protein rice prolamin

Y. R. Mawal, P. K. Ranjekar, U. P. Shinde, D. B. Akolekar, V. R. Choudhary

Research output: Contribution to journalArticle

Abstract

A simple and easy affinity purification of antibodies raised against a highly hydrophobic protein is described. This method employs silicalite zeolite, a solid matrix, to which prolamin is adsorbed. A rapid and sensitive immunoradiometric assay was developed to quantitate prolamin. Maximum prolamin in rice is present between 10 to 14 days post-anthesis.

Original languageEnglish (US)
Pages (from-to)177-180
Number of pages4
JournalBiotechnology Techniques
Volume4
Issue number3
DOIs
StatePublished - May 1 1990

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ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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