Silicalite zeolite: A novel matrix for purification and estimation of a hydrophobic protein rice prolamin

Y. R. Mawal, P. K. Ranjekar, Ujwal Shinde, D. B. Akolekar, V. R. Choudhary

Research output: Contribution to journalArticle

Abstract

A simple and easy affinity purification of antibodies raised against a highly hydrophobic protein is described. This method employs silicalite zeolite, a solid matrix, to which prolamin is adsorbed. A rapid and sensitive immunoradiometric assay was developed to quantitate prolamin. Maximum prolamin in rice is present between 10 to 14 days post-anthesis.

Original languageEnglish (US)
Pages (from-to)177-180
Number of pages4
JournalBiotechnology Techniques
Volume4
Issue number3
DOIs
StatePublished - May 1990
Externally publishedYes

Fingerprint

Prolamins
Zeolites
Antibodies
Purification
Assays
Proteins
Immunoradiometric Assay
Antibody Affinity
Oryza

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Silicalite zeolite : A novel matrix for purification and estimation of a hydrophobic protein rice prolamin. / Mawal, Y. R.; Ranjekar, P. K.; Shinde, Ujwal; Akolekar, D. B.; Choudhary, V. R.

In: Biotechnology Techniques, Vol. 4, No. 3, 05.1990, p. 177-180.

Research output: Contribution to journalArticle

Mawal, Y. R. ; Ranjekar, P. K. ; Shinde, Ujwal ; Akolekar, D. B. ; Choudhary, V. R. / Silicalite zeolite : A novel matrix for purification and estimation of a hydrophobic protein rice prolamin. In: Biotechnology Techniques. 1990 ; Vol. 4, No. 3. pp. 177-180.
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