Silencing the type II sodium channel gene

A model for neural-specific gene regulation

Susan D. Kraner, Jayhong A. Chong, Huey Jen Tsay, Gail Mandel

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

Neural-specific expression of a sodium channel minigene has been shown to be mediated by a 28 bp silencer element, RE1, located in the 5′ flanking region of the gene. This element is active exclusively in cell lines that do not express the endogenous brain type II sodium channel gene, including fibroblast, skeletal muscle, and certain neuronal cell lines. All of these non-type II expressing cells contain RE1-binding complexes. On the basis of mutational analysis and in vivo "repressor trap" experiments, we propose that cell-specific RE1-binding proteins are responsible, at least in part, for restricting expression of the type II sodium channel gene to specific neurons in the vertebrate nervous system.

Original languageEnglish (US)
Pages (from-to)37-44
Number of pages8
JournalNeuron
Volume9
Issue number1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Sodium Channels
Transcriptional Silencer Elements
Genes
Cell Line
5' Flanking Region
Nervous System
Vertebrates
Carrier Proteins
Skeletal Muscle
Fibroblasts
Neurons
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Silencing the type II sodium channel gene : A model for neural-specific gene regulation. / Kraner, Susan D.; Chong, Jayhong A.; Tsay, Huey Jen; Mandel, Gail.

In: Neuron, Vol. 9, No. 1, 1992, p. 37-44.

Research output: Contribution to journalArticle

Kraner, Susan D. ; Chong, Jayhong A. ; Tsay, Huey Jen ; Mandel, Gail. / Silencing the type II sodium channel gene : A model for neural-specific gene regulation. In: Neuron. 1992 ; Vol. 9, No. 1. pp. 37-44.
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