TY - JOUR
T1 - Silencing the type II sodium channel gene
T2 - A model for neural-specific gene regulation
AU - Kraner, Susan D.
AU - Chong, Jayhong A.
AU - Tsay, Huey Jen
AU - Mandel, Gail
N1 - Funding Information:
The authors thank Drs. 5. Halegoua, R. H. Goodman, and S. Strickland for critical reading of the manuscript. The technical contributions and advice of Kimberly Gilmour, Navid Kazemi, Jit Ray, and Yingcong Zheng are also gratefully acknowledged. The research was supported by a National Institutes of Health grant to G. M. and an NRSA award to S. D. K.
PY - 1992/7
Y1 - 1992/7
N2 - Neural-specific expression of a sodium channel minigene has been shown to be mediated by a 28 bp silencer element, RE1, located in the 5′ flanking region of the gene. This element is active exclusively in cell lines that do not express the endogenous brain type II sodium channel gene, including fibroblast, skeletal muscle, and certain neuronal cell lines. All of these non-type II expressing cells contain RE1-binding complexes. On the basis of mutational analysis and in vivo "repressor trap" experiments, we propose that cell-specific RE1-binding proteins are responsible, at least in part, for restricting expression of the type II sodium channel gene to specific neurons in the vertebrate nervous system.
AB - Neural-specific expression of a sodium channel minigene has been shown to be mediated by a 28 bp silencer element, RE1, located in the 5′ flanking region of the gene. This element is active exclusively in cell lines that do not express the endogenous brain type II sodium channel gene, including fibroblast, skeletal muscle, and certain neuronal cell lines. All of these non-type II expressing cells contain RE1-binding complexes. On the basis of mutational analysis and in vivo "repressor trap" experiments, we propose that cell-specific RE1-binding proteins are responsible, at least in part, for restricting expression of the type II sodium channel gene to specific neurons in the vertebrate nervous system.
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U2 - 10.1016/0896-6273(92)90218-3
DO - 10.1016/0896-6273(92)90218-3
M3 - Article
C2 - 1321645
AN - SCOPUS:0026659651
SN - 0896-6273
VL - 9
SP - 37
EP - 44
JO - Neuron
JF - Neuron
IS - 1
ER -