Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo.

David L. Gillespie, Jeannette R. Flynn, Brian T. Ragel, Maria Arce-Larreta, David A. Kelly, Sheryl R. Tripp, Randy L. Jensen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Higher-grade gliomas are distinguished by increased vascular endothelial cell proliferation and peritumoral edema. These are thought to be instigated by vascular endothelial growth factor, which in turn is regulated by cellular oxygen tension. Hypoxia inducible factor-1alpha (HIF-1alpha) is a main responder to intracellular hypoxia and is overexpressed in many human cancers, including gliomas. Here we present methods for investigating the role of HIF-1alpha in glioma growth in vivo and in vitro using RNA interference in U251, U87, and U373 glioma cells.

Original languageEnglish (US)
Pages (from-to)283-301
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume487
StatePublished - 2009
Externally publishedYes

Fingerprint

RNA Interference
Glioma
Vascular Endothelial Growth Factor A
Edema
Endothelial Cells
Cell Proliferation
Oxygen
Hypoxia
In Vitro Techniques
Growth
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gillespie, D. L., Flynn, J. R., Ragel, B. T., Arce-Larreta, M., Kelly, D. A., Tripp, S. R., & Jensen, R. L. (2009). Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo. Methods in molecular biology (Clifton, N.J.), 487, 283-301.

Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo. / Gillespie, David L.; Flynn, Jeannette R.; Ragel, Brian T.; Arce-Larreta, Maria; Kelly, David A.; Tripp, Sheryl R.; Jensen, Randy L.

In: Methods in molecular biology (Clifton, N.J.), Vol. 487, 2009, p. 283-301.

Research output: Contribution to journalArticle

Gillespie, DL, Flynn, JR, Ragel, BT, Arce-Larreta, M, Kelly, DA, Tripp, SR & Jensen, RL 2009, 'Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo.', Methods in molecular biology (Clifton, N.J.), vol. 487, pp. 283-301.
Gillespie DL, Flynn JR, Ragel BT, Arce-Larreta M, Kelly DA, Tripp SR et al. Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo. Methods in molecular biology (Clifton, N.J.). 2009;487:283-301.
Gillespie, David L. ; Flynn, Jeannette R. ; Ragel, Brian T. ; Arce-Larreta, Maria ; Kelly, David A. ; Tripp, Sheryl R. ; Jensen, Randy L. / Silencing of HIF-1alpha by RNA interference in human glioma cells in vitro and in vivo. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 487. pp. 283-301.
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