Significant downregulation of platelet gene expression in metastatic lung cancer

David C. Calverley, Tzu L. Phang, Qamrul G. Choudhury, Bifeng Gao, Ana B. Oton, Michael J. Weyant, Mark W. Geraci

    Research output: Contribution to journalArticle

    39 Scopus citations

    Abstract

    Platelets play a major role in the metastatic dissemination of tumor cells in vivo. Recent evidence reveals megakaryocyte-derived platelet pre-mRNA is spliced to mRNA and then translated into functional proteins in response to external stimulation. Employing a human lung cancer model, we hypothesized a subset of megakaryocyte/platelet genes exists that are significantly over or underexpressed in metastasis compared with noncancer. Microarray analysis employing platelet mRNA followed by unsupervised hierarchical clustering revealed an expression profile that includes decreased expression of 197 of the 200 platelet genes with the most altered expression (p < 1.0 × 10-4). Among the 608 splicing events identified between the metastasis and negative control groups, 33 highly variable genes were identified with between 3 and 13 splicing events each. In conclusion, this preliminary study reveals a platelet-based gene expression signature that differentiates metastatic lung cancer from negative controls on the basis of decreased expression of 197 of the 200 genes with the most altered expression levels. Further study may yield a prognostic tool for future metastasis among subsets of early stage lung cancer patients.

    Original languageEnglish (US)
    Pages (from-to)227-232
    Number of pages6
    JournalClinical and Translational Science
    Volume3
    Issue number5
    DOIs
    StatePublished - Oct 2010

    Keywords

    • Lung cancer
    • Metastasis
    • Platelets

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Calverley, D. C., Phang, T. L., Choudhury, Q. G., Gao, B., Oton, A. B., Weyant, M. J., & Geraci, M. W. (2010). Significant downregulation of platelet gene expression in metastatic lung cancer. Clinical and Translational Science, 3(5), 227-232. https://doi.org/10.1111/j.1752-8062.2010.00226.x