Signaling pathways implicated in androgen regulation of endocortical bone

Kristine Wiren, Anthony A. Semirale, Joel G. Hashimoto, Xiao Wei Zhang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Periosteal expansion is a recognized response to androgen exposure during bone development and in profoundly hypogonadal adults. However, androgen also suppresses endocortical bone formation, indicating that its effects on bone are dichotomous and envelope-specific. In fact, enhanced androgen signaling has been shown to have dramatic detrimental effects on whole bone biomechanical properties in two different transgenic models with skeletally targeted androgen receptor (AR) overexpression. As the mechanisms underlying this response are uncharacterized, we compared patterns of gene expression in periosteum-free cortical bone samples derived from AR-overexpressing transgenic male mice and their wild-type counterparts. We then assessed direct androgen effects in both wild-type and AR-overexpressing osteoblasts in primary culture. Among major signaling pathways associated with bone formation, focused quantitative RT-PCR (qPCR) array-based analysis of endocortical bone gene expression from wild-type vs. transgenic males identified the transforming growth factor-beta (TGF-Β) superfamily and bone morphogenetic protein (BMP) signaling as significantly altered by androgen in vivo. Bioinformatic analyses indicated proliferation, osteoblast differentiation and mineralization as major biological processes affected. Consistent with the in vivo array data and bioinformatic analyses, inhibition of differentiation observed with androgen exposure was reduced by exogenous BMP2 treatment of AR-overexpressing cultures to stimulate BMP signaling, confirming array pathway analysis. In addition, nonaromatizable dihydrotestosterone (DHT) inhibited osteoblast proliferation, differentiation and several indices of mineralization, including mineral accumulation and mineralized nodule formation in primary cultures from both wild-type and AR-transgenic mice. These findings identify a molecular mechanism based on altered BMP signaling that contributes to androgen inhibition of osteoblast differentiation and mineralization. Such detrimental effects of androgen on osteoblast function may underlie the generally disappointing results of androgen therapy.

Original languageEnglish (US)
Pages (from-to)710-723
Number of pages14
JournalBone
Volume46
Issue number3
DOIs
StatePublished - Mar 2010

Fingerprint

Androgens
Bone and Bones
Androgen Receptors
Osteoblasts
Bone Morphogenetic Proteins
Computational Biology
Osteogenesis
Transgenic Mice
Biological Phenomena
Gene Expression
Periosteum
Dihydrotestosterone
Bone Development
Transforming Growth Factor beta
Minerals
Polymerase Chain Reaction

Keywords

  • Androgen
  • Androgen receptor
  • BMP
  • Bone formation
  • Osteoblast
  • TGF-Β

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology
  • Medicine(all)

Cite this

Signaling pathways implicated in androgen regulation of endocortical bone. / Wiren, Kristine; Semirale, Anthony A.; Hashimoto, Joel G.; Zhang, Xiao Wei.

In: Bone, Vol. 46, No. 3, 03.2010, p. 710-723.

Research output: Contribution to journalArticle

Wiren, Kristine ; Semirale, Anthony A. ; Hashimoto, Joel G. ; Zhang, Xiao Wei. / Signaling pathways implicated in androgen regulation of endocortical bone. In: Bone. 2010 ; Vol. 46, No. 3. pp. 710-723.
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