Shuttle mutagenesis of Neisseria gonorrhoeae

Pilin null mutations lower DNA transformation competence

H. S. Seifert, R. S. Ajioka, D. Paruchuri, Fred Heffron, M. So

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibiotic resistance in N. gonorrhoeae. Isogenic N. gonorrhoeae strains that differ only in pilin expression were constructed and used to test the effect of pilin null mutations on DNA transformation competence.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalJournal of Bacteriology
Volume172
Issue number1
StatePublished - 1990
Externally publishedYes

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DNA Transformation Competence
Fimbriae Proteins
Neisseria gonorrhoeae
Mutagenesis
Chloramphenicol Resistance
Mutation
Microbial Drug Resistance
Escherichia coli
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Seifert, H. S., Ajioka, R. S., Paruchuri, D., Heffron, F., & So, M. (1990). Shuttle mutagenesis of Neisseria gonorrhoeae: Pilin null mutations lower DNA transformation competence. Journal of Bacteriology, 172(1), 40-46.

Shuttle mutagenesis of Neisseria gonorrhoeae : Pilin null mutations lower DNA transformation competence. / Seifert, H. S.; Ajioka, R. S.; Paruchuri, D.; Heffron, Fred; So, M.

In: Journal of Bacteriology, Vol. 172, No. 1, 1990, p. 40-46.

Research output: Contribution to journalArticle

Seifert, HS, Ajioka, RS, Paruchuri, D, Heffron, F & So, M 1990, 'Shuttle mutagenesis of Neisseria gonorrhoeae: Pilin null mutations lower DNA transformation competence', Journal of Bacteriology, vol. 172, no. 1, pp. 40-46.
Seifert, H. S. ; Ajioka, R. S. ; Paruchuri, D. ; Heffron, Fred ; So, M. / Shuttle mutagenesis of Neisseria gonorrhoeae : Pilin null mutations lower DNA transformation competence. In: Journal of Bacteriology. 1990 ; Vol. 172, No. 1. pp. 40-46.
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