Short-term depression of sprouted mossy fiber synapses from adult-born granule cells

William D. Hendricks, Yang Chen, Aesoon L. Bensen, Gary Westbrook, Eric Schnell

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Epileptic seizures potently modulate hippocampal adult neurogenesis, and adult-born dentate granule cells contribute to the pathologic retrograde sprouting of mossy fiber axons, both hallmarks of temporal lobe epilepsy. The characteristics of these sprouted synapses, however, have been largely unexplored, and the specific contribution of adult-born granule cells to functional mossy fiber sprouting is unknown, primarily due to technical barriers in isolating sprouted mossy fiber synapses for analysis. Here, we used DcxCreERT2 transgenic mice to permanently pulse-label age-defined cohorts of granule cells born either before or after pilocarpine-induced status epilepticus (SE). Using optogenetics, we demonstrate that adult-born granule cells born before SE form functional recurrent monosynaptic excitatory connections with other granule cells. Surprisingly, however, although healthy mossy fiber synapses in CA3 are well characterized “detonator” synapses that potently drive postsynaptic cell firing through their profound frequency-dependent facilitation, sprouted mossy fiber synapses from adult-born cells exhibited profound frequency-dependent depression, despite possessing some of the morphological hallmarks of mossy fiber terminals. Mature granule cells also contributed to functional mossy fiber sprouting, but exhibited less synaptic depression. Interestingly, granule cells born shortly after SE did not form functional excitatory synapses, despite robust sprouting. Our results suggest that, although sprouted mossy fibers form recurrent excitatory circuits with some of the morphological characteristics of typical mossy fiber terminals, the functional characteristics of sprouted synapses would limit the contribution of adult-born granule cells to hippocampal hyperexcitability in the epileptic hippocampus.

Original languageEnglish (US)
Pages (from-to)5722-5735
Number of pages14
JournalJournal of Neuroscience
Volume37
Issue number23
DOIs
StatePublished - Jun 7 2017

Fingerprint

Synapses
Status Epilepticus
Optogenetics
Pilocarpine
Temporal Lobe Epilepsy
Neurogenesis
Transgenic Mice
Axons
Epilepsy
Hippocampus

Keywords

  • Adult neurogenesis
  • Dentate
  • Epilepsy
  • Mossy fiber
  • Seizure
  • Sprouting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Short-term depression of sprouted mossy fiber synapses from adult-born granule cells. / Hendricks, William D.; Chen, Yang; Bensen, Aesoon L.; Westbrook, Gary; Schnell, Eric.

In: Journal of Neuroscience, Vol. 37, No. 23, 07.06.2017, p. 5722-5735.

Research output: Contribution to journalArticle

Hendricks, William D. ; Chen, Yang ; Bensen, Aesoon L. ; Westbrook, Gary ; Schnell, Eric. / Short-term depression of sprouted mossy fiber synapses from adult-born granule cells. In: Journal of Neuroscience. 2017 ; Vol. 37, No. 23. pp. 5722-5735.
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