Short- and long-term behavioral effects of exposure to 21%, 40% and 100% oxygen after perinatal hypoxia-ischemia in the rat

K. Nina Woodworth, Julie Palmateer, Joseph Swide, Marjorie Grafe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Until recently, supplementation with 100% oxygen was standard therapy for newborns who required resuscitation at birth or suffered later hypoxic-ischemic events. Exposure to high concentrations of oxygen, however, may worsen oxidative stress induced by ischemic injury. In this study we investigated the short- and long-term behavioral outcomes in rats that had undergone hypoxic-ischemic brain injury on postnatal day 7, followed by 2. h exposure to 21%, 40%, or 100% oxygen, compared to normal controls. There were no differences in the development of walking, head lifting and righting reflexes from postnatal days 9 to 15. Cliff avoidance showed some abnormal responses in the H21 animals. From postnatal days 28 to 56, three tests of sensorimotor coordination were performed weekly: ledged tapered beam, cylinder, and bilateral tactile stimulation. The ledged tapered beam test without prior training of animals was sensitive to injury, but did not distinguish between treatment groups. The cylinder test showed a greater use of the unimpaired limb in female 21% and 40% oxygen groups compared to controls. Performance in both cylinder and the beam tests showed a correlation with the degree of brain injury. The bilateral tactile stimulation test showed that the male 21% oxygen groups had worse sensory asymmetry than male 40% or 100% oxygen groups, but was not statistically significantly different from controls.We thus found a minor benefit to post-hypoxia-ischemic treatment with 100% and 40% oxygen compared to 21% in one test of early motor skills. Our results for long-term sensorimotor behavior, however, showed conflicting results, however, as males treated with 40% or 100% oxygen had less sensory asymmetry (better performance) in the bilateral tactile stimulation test than males treated with 21% oxygen, while females had impaired motor performance in the cylinder test with both 21% and 40% oxygen.

Original languageEnglish (US)
Pages (from-to)629-638
Number of pages10
JournalInternational Journal of Developmental Neuroscience
Volume29
Issue number6
DOIs
StatePublished - Oct 2011

Fingerprint

Ischemia
Oxygen
Touch
Brain Injuries
Hypoxia
Righting Reflex
Motor Skills
Wounds and Injuries
Resuscitation
Walking
Oxidative Stress
Therapeutics
Extremities
Head
Parturition

Keywords

  • Behavior
  • Oxygen
  • Perinatal hypoxia-ischemia
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Short- and long-term behavioral effects of exposure to 21%, 40% and 100% oxygen after perinatal hypoxia-ischemia in the rat. / Woodworth, K. Nina; Palmateer, Julie; Swide, Joseph; Grafe, Marjorie.

In: International Journal of Developmental Neuroscience, Vol. 29, No. 6, 10.2011, p. 629-638.

Research output: Contribution to journalArticle

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abstract = "Until recently, supplementation with 100{\%} oxygen was standard therapy for newborns who required resuscitation at birth or suffered later hypoxic-ischemic events. Exposure to high concentrations of oxygen, however, may worsen oxidative stress induced by ischemic injury. In this study we investigated the short- and long-term behavioral outcomes in rats that had undergone hypoxic-ischemic brain injury on postnatal day 7, followed by 2. h exposure to 21{\%}, 40{\%}, or 100{\%} oxygen, compared to normal controls. There were no differences in the development of walking, head lifting and righting reflexes from postnatal days 9 to 15. Cliff avoidance showed some abnormal responses in the H21 animals. From postnatal days 28 to 56, three tests of sensorimotor coordination were performed weekly: ledged tapered beam, cylinder, and bilateral tactile stimulation. The ledged tapered beam test without prior training of animals was sensitive to injury, but did not distinguish between treatment groups. The cylinder test showed a greater use of the unimpaired limb in female 21{\%} and 40{\%} oxygen groups compared to controls. Performance in both cylinder and the beam tests showed a correlation with the degree of brain injury. The bilateral tactile stimulation test showed that the male 21{\%} oxygen groups had worse sensory asymmetry than male 40{\%} or 100{\%} oxygen groups, but was not statistically significantly different from controls.We thus found a minor benefit to post-hypoxia-ischemic treatment with 100{\%} and 40{\%} oxygen compared to 21{\%} in one test of early motor skills. Our results for long-term sensorimotor behavior, however, showed conflicting results, however, as males treated with 40{\%} or 100{\%} oxygen had less sensory asymmetry (better performance) in the bilateral tactile stimulation test than males treated with 21{\%} oxygen, while females had impaired motor performance in the cylinder test with both 21{\%} and 40{\%} oxygen.",
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