Shc phosphorylation in myeloid cells is regulated by granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor and is constitutively increased by p210BCR/ABL

Tetsuya Matsuguchi, Ravi Salgia, Michael Hallek, Matthias Eder, Brian Druker, Timothy J. Ernst, James D. Griffin

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor induce proliferation of hematopoietic cells through binding to specific, high affinity, cell surface receptors. However, little is known about post-receptor signal transduction pathways. Here we report that an SH2 domain containing protein previously implicated in the activation of p21ras, Shc, is transiently tyrosine phosphorylated in myeloid cells after stimulation with granulocyte macrophage colonystimulating factor, interleukin-3, or steel factor. Also, She was found to be constitutively tyrosine phosphorylated in myeloid cell lines made factor independent by expression of p210BCR/ABL. A Shc-associated 140-kDa protein was identified, which was phosphorylated on tyrosine residues transiently after cytokine stimulation and constitutively after expression of p210BCR/ABL. These findings suggest that Shc could play an important role in a signal transduction pathway, which leads to the proliferation of myeloid cells.

Original languageEnglish (US)
Pages (from-to)5016-5021
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number7
StatePublished - Feb 18 1994
Externally publishedYes

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Phosphorylation
Stem Cell Factor
Interleukin-3
Myeloid Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Tyrosine
Signal transduction
Signal Transduction
src Homology Domains
Macrophages
Cell Surface Receptors
Granulocytes
Proteins
Chemical activation
Cells
Cell Proliferation
Cytokines
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Shc phosphorylation in myeloid cells is regulated by granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor and is constitutively increased by p210BCR/ABL . / Matsuguchi, Tetsuya; Salgia, Ravi; Hallek, Michael; Eder, Matthias; Druker, Brian; Ernst, Timothy J.; Griffin, James D.

In: Journal of Biological Chemistry, Vol. 269, No. 7, 18.02.1994, p. 5016-5021.

Research output: Contribution to journalArticle

Matsuguchi, Tetsuya ; Salgia, Ravi ; Hallek, Michael ; Eder, Matthias ; Druker, Brian ; Ernst, Timothy J. ; Griffin, James D. / Shc phosphorylation in myeloid cells is regulated by granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor and is constitutively increased by p210BCR/ABL . In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 7. pp. 5016-5021.
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AU - Hallek, Michael

AU - Eder, Matthias

AU - Druker, Brian

AU - Ernst, Timothy J.

AU - Griffin, James D.

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