Shc phosphorylation in myeloid cells is regulated by granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor and is constitutively increased by p210(BCR/ABL)

T. Matsuguchi, R. Salgia, M. Hallek, M. Eder, B. Druker, T. J. Ernst, J. D. Griffin

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

Granulocyte macrophage colony-stimulating factor, interleukin-3, and steel factor induce proliferation of hematopoietic cells through binding to specific, high affinity, cell surface receptors. However, little is known about post-receptor signal transduction pathways. Here we report that an SH2 domain containing protein previously implicated in the activation of p21(ras), Shc, is transiently tyrosine phosphorylated in myeloid cells after stimulation with granulocyte macrophage colony-stimulating factor, interleukin-3, or steel factor. Also, Shc was found to be constitutively tyrosine phosphorylated in myeloid cell lines made factor independent by expression of p210(BCR/ABL). A Shc-associated 140-kDa protein was identified, which was phosphorylated on tyrosine residues transiently after cytokine stimulation and constitutively after expression of p210(BCR/ABL). These findings suggest that Shc could play an important role in a signal transduction pathway, which leads to the proliferation of myeloid cells.

Original languageEnglish (US)
Pages (from-to)5016-5021
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number7
StatePublished - Jan 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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