Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats

Richard G. Bayles, Joanne Tran, Antoinette Olivas, William Woodward, Suzanne S. Fei, Lina Gao, Beth Habecker

Research output: Contribution to journalArticle

Abstract

The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of sympathetic nerves projecting to the heart has been observed in several cardiovascular diseases, and sympathetic dysfunction contributes to cardiac pathology. Wistar Kyoto rats are a common model for the study of cardiovascular diseases, but we lack a profile of the baseline transcriptomic and neurochemical characteristics of their cardiac sympathetic neurons. Most studies of cardiovascular disease have used male animals only, but in the future both male and female animals will be used for these types of studies; therefore, we sought to characterize the transcriptome of male and female stellate ganglia and to correlate that with catecholamine and acetylcholine content in the heart. We have generated a dataset of baseline RNA expression in male and female Wistar Kyoto rat stellate ganglia using RNA-seq, and have measured neurotransmitter levels in heart and stellate ganglia using HPLC and mass spectrometry. We identified numerous gene expression differences between male and female stellates, including genes encoding important developmental factors, receptors and neuropeptides. Female hearts had significantly higher neurotransmitter content than male hearts; however, no significant differences were detected in expression of the genes encoding neurotransmitter synthetic enzymes. Similarly, no statistically significant differences were identified between the sexes in cardiac tyrosine hydroxylase levels.

Original languageEnglish (US)
Article numbere0218133
JournalPloS one
Volume14
Issue number6
DOIs
StatePublished - Jun 1 2019

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Neurochemistry
Inbred WKY Rats
Gene expression
gender differences
Sex Characteristics
Stellate Ganglion
Rats
heart
Gene Expression
gene expression
rats
neurotransmitters
cardiovascular diseases
Neurotransmitter Agents
Gene encoding
Cardiovascular Diseases
tyrosine 3-monooxygenase
Animals
Neuropeptide Receptors
RNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats. / Bayles, Richard G.; Tran, Joanne; Olivas, Antoinette; Woodward, William; Fei, Suzanne S.; Gao, Lina; Habecker, Beth.

In: PloS one, Vol. 14, No. 6, e0218133, 01.06.2019.

Research output: Contribution to journalArticle

Bayles, Richard G. ; Tran, Joanne ; Olivas, Antoinette ; Woodward, William ; Fei, Suzanne S. ; Gao, Lina ; Habecker, Beth. / Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats. In: PloS one. 2019 ; Vol. 14, No. 6.
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