Sex differences in activation of the hypothalamic-pituitary-adrenal axis by methamphetamine

Damian G. Zuloaga, Lance A. Johnson, Maayan Agam, Jacob Raber

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Dysregulation of hypothalamic-pituitary-adrenal (HPA) axis activation is associated with changes in addiction-related behaviors. In this study, we tested whether sex differences in the acute effects of methamphetamine (MA) exposure involve differential activation of the HPA axis. Male and female mice were injected with MA (1 mg/kg) or saline for comparison of plasma corticosterone and analysis of the immediate early gene c-Fos in brain. There was a prolonged elevation in corticosterone levels in female compared to male mice. C-Fos was elevated in both sexes following MA in HPA axis-associated regions, including the hypothalamic paraventricular nucleus (PVN), central amygdala, cingulate, and CA3 hippocampal region. MA increased the number of c-Fos and c-Fos/glucocorticoid receptor (GR) dual-labeled cells to a greater extent in males than females in the cingulate and CA3 regions. MA also increased the number of c-fos/vasopressin dual-labeled cells in the PVN as well as the number and percentage of c-Fos/GR dual-labeled cells in the PVN and central amygdala, although no sex differences in dual labeling were found in these regions. Thus, sex differences in MA-induced plasma corticosterone levels and activation of distinct brain regions and proteins involved in HPA axis regulation may contribute to sex differences in acute effects of MA on the brain.

Original languageEnglish (US)
Pages (from-to)495-508
Number of pages14
JournalJournal of Neurochemistry
Volume129
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Methamphetamine
Sex Characteristics
Chemical activation
Paraventricular Hypothalamic Nucleus
Corticosterone
Brain
Hippocampal CA3 Region
Plasmas
Immediate-Early Genes
Gyrus Cinguli
Glucocorticoid Receptors
Amygdala
Vasopressins
Labeling
Genes

Keywords

  • arginine vasopressin glucocorticoid receptor
  • HPA axis corticosterone
  • methamphetamine
  • sex difference

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Sex differences in activation of the hypothalamic-pituitary-adrenal axis by methamphetamine. / Zuloaga, Damian G.; Johnson, Lance A.; Agam, Maayan; Raber, Jacob.

In: Journal of Neurochemistry, Vol. 129, No. 3, 2014, p. 495-508.

Research output: Contribution to journalArticle

Zuloaga, Damian G. ; Johnson, Lance A. ; Agam, Maayan ; Raber, Jacob. / Sex differences in activation of the hypothalamic-pituitary-adrenal axis by methamphetamine. In: Journal of Neurochemistry. 2014 ; Vol. 129, No. 3. pp. 495-508.
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