Several GABA(A) receptor subunits are expressed in LHRH neurons of juvenile female rats

Heike Jung, Eva M. Shannon, Jean Marc Fritschy, Sergio Ojeda

    Research output: Contribution to journalArticle

    81 Citations (Scopus)

    Abstract

    Gamma aminobutyric acid (GABA), the dominant inhibitory neurotransmitter in brain, is involved in the developmental regulation of LHRH secretion. Morphological studies in rodents have demonstrated that LHRH neurons are innervated by GABA-containing processes, suggesting that LHRH secretion is under direct transsynaptic GABAergic control. While GABA acts through two different receptors, GABA(A) and GABA(B), to exert its effects, it appears that GABA(A) receptors are able to mediate both inhibitory and stimulatory effects of GABA on LHRH neurons. GABA(A) receptors are heterooligomeric ligand-gated anion channels that exhibit a diverse array of functional and pharmacological properties. This diversity is determined by the structural heterogeneity of the receptors, which are assembled from the combination of different classes of subunits with multiple isoforms. Although several studies have described the effect of GABA(A) receptor stimulation on LHRH and/or gonadotropin release in prepubertal animals, nothing is known about the receptor subunits that may be expressed in LHRH neurons at this phase in development. Double immunohistofluorescence followed by confocal laser microscopy revealed that subsets of prepubertal LHRH neurons are endowed with α1, α2, β(2/3), and γ2 GABA(A) receptor subunits. Combined immunohistochemistry for LHRH neurons and in situ hybridization for GABA(A) subunit mRNAs confirmed that the genes encoding the α1, α2, β3, and γ2 subnnits, but not the γ1 subunit, are expressed in LHRH neurons. Notwithstanding the relative insensitivity of these methods, both the immunohistochemical and hybridization histochemical approaches employed indicate that only a fraction of LHRH neurons are endowed with GABA(A) receptors. This arrangement suggests that those LHRH neurons bearing the appropriate GABA(A) receptors are responsible for either the entire secretory response to direct GABAergic inputs or for its initiation.

    Original languageEnglish (US)
    Pages (from-to)218-229
    Number of pages12
    JournalBrain Research
    Volume780
    Issue number2
    DOIs
    StatePublished - Jan 12 1998

    Fingerprint

    GABA Receptors
    Gonadotropin-Releasing Hormone
    Neurons
    gamma-Aminobutyric Acid
    Confocal Microscopy
    Ligand-Gated Ion Channels
    Gonadotropins
    In Situ Hybridization
    Anions
    Neurotransmitter Agents
    Rodentia
    Protein Isoforms
    Immunohistochemistry
    Pharmacology

    Keywords

    • GABA(A) receptor
    • Hypothalamus
    • Immunohistochemistry
    • In situ hybridization
    • Sexual development

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Clinical Neurology
    • Developmental Biology
    • Molecular Biology

    Cite this

    Several GABA(A) receptor subunits are expressed in LHRH neurons of juvenile female rats. / Jung, Heike; Shannon, Eva M.; Fritschy, Jean Marc; Ojeda, Sergio.

    In: Brain Research, Vol. 780, No. 2, 12.01.1998, p. 218-229.

    Research output: Contribution to journalArticle

    Jung, Heike ; Shannon, Eva M. ; Fritschy, Jean Marc ; Ojeda, Sergio. / Several GABA(A) receptor subunits are expressed in LHRH neurons of juvenile female rats. In: Brain Research. 1998 ; Vol. 780, No. 2. pp. 218-229.
    @article{abbcca18e688482fbc0f514a9482213c,
    title = "Several GABA(A) receptor subunits are expressed in LHRH neurons of juvenile female rats",
    abstract = "Gamma aminobutyric acid (GABA), the dominant inhibitory neurotransmitter in brain, is involved in the developmental regulation of LHRH secretion. Morphological studies in rodents have demonstrated that LHRH neurons are innervated by GABA-containing processes, suggesting that LHRH secretion is under direct transsynaptic GABAergic control. While GABA acts through two different receptors, GABA(A) and GABA(B), to exert its effects, it appears that GABA(A) receptors are able to mediate both inhibitory and stimulatory effects of GABA on LHRH neurons. GABA(A) receptors are heterooligomeric ligand-gated anion channels that exhibit a diverse array of functional and pharmacological properties. This diversity is determined by the structural heterogeneity of the receptors, which are assembled from the combination of different classes of subunits with multiple isoforms. Although several studies have described the effect of GABA(A) receptor stimulation on LHRH and/or gonadotropin release in prepubertal animals, nothing is known about the receptor subunits that may be expressed in LHRH neurons at this phase in development. Double immunohistofluorescence followed by confocal laser microscopy revealed that subsets of prepubertal LHRH neurons are endowed with α1, α2, β(2/3), and γ2 GABA(A) receptor subunits. Combined immunohistochemistry for LHRH neurons and in situ hybridization for GABA(A) subunit mRNAs confirmed that the genes encoding the α1, α2, β3, and γ2 subnnits, but not the γ1 subunit, are expressed in LHRH neurons. Notwithstanding the relative insensitivity of these methods, both the immunohistochemical and hybridization histochemical approaches employed indicate that only a fraction of LHRH neurons are endowed with GABA(A) receptors. This arrangement suggests that those LHRH neurons bearing the appropriate GABA(A) receptors are responsible for either the entire secretory response to direct GABAergic inputs or for its initiation.",
    keywords = "GABA(A) receptor, Hypothalamus, Immunohistochemistry, In situ hybridization, Sexual development",
    author = "Heike Jung and Shannon, {Eva M.} and Fritschy, {Jean Marc} and Sergio Ojeda",
    year = "1998",
    month = "1",
    day = "12",
    doi = "10.1016/S0006-8993(97)01152-9",
    language = "English (US)",
    volume = "780",
    pages = "218--229",
    journal = "Brain Research",
    issn = "0006-8993",
    publisher = "Elsevier",
    number = "2",

    }

    TY - JOUR

    T1 - Several GABA(A) receptor subunits are expressed in LHRH neurons of juvenile female rats

    AU - Jung, Heike

    AU - Shannon, Eva M.

    AU - Fritschy, Jean Marc

    AU - Ojeda, Sergio

    PY - 1998/1/12

    Y1 - 1998/1/12

    N2 - Gamma aminobutyric acid (GABA), the dominant inhibitory neurotransmitter in brain, is involved in the developmental regulation of LHRH secretion. Morphological studies in rodents have demonstrated that LHRH neurons are innervated by GABA-containing processes, suggesting that LHRH secretion is under direct transsynaptic GABAergic control. While GABA acts through two different receptors, GABA(A) and GABA(B), to exert its effects, it appears that GABA(A) receptors are able to mediate both inhibitory and stimulatory effects of GABA on LHRH neurons. GABA(A) receptors are heterooligomeric ligand-gated anion channels that exhibit a diverse array of functional and pharmacological properties. This diversity is determined by the structural heterogeneity of the receptors, which are assembled from the combination of different classes of subunits with multiple isoforms. Although several studies have described the effect of GABA(A) receptor stimulation on LHRH and/or gonadotropin release in prepubertal animals, nothing is known about the receptor subunits that may be expressed in LHRH neurons at this phase in development. Double immunohistofluorescence followed by confocal laser microscopy revealed that subsets of prepubertal LHRH neurons are endowed with α1, α2, β(2/3), and γ2 GABA(A) receptor subunits. Combined immunohistochemistry for LHRH neurons and in situ hybridization for GABA(A) subunit mRNAs confirmed that the genes encoding the α1, α2, β3, and γ2 subnnits, but not the γ1 subunit, are expressed in LHRH neurons. Notwithstanding the relative insensitivity of these methods, both the immunohistochemical and hybridization histochemical approaches employed indicate that only a fraction of LHRH neurons are endowed with GABA(A) receptors. This arrangement suggests that those LHRH neurons bearing the appropriate GABA(A) receptors are responsible for either the entire secretory response to direct GABAergic inputs or for its initiation.

    AB - Gamma aminobutyric acid (GABA), the dominant inhibitory neurotransmitter in brain, is involved in the developmental regulation of LHRH secretion. Morphological studies in rodents have demonstrated that LHRH neurons are innervated by GABA-containing processes, suggesting that LHRH secretion is under direct transsynaptic GABAergic control. While GABA acts through two different receptors, GABA(A) and GABA(B), to exert its effects, it appears that GABA(A) receptors are able to mediate both inhibitory and stimulatory effects of GABA on LHRH neurons. GABA(A) receptors are heterooligomeric ligand-gated anion channels that exhibit a diverse array of functional and pharmacological properties. This diversity is determined by the structural heterogeneity of the receptors, which are assembled from the combination of different classes of subunits with multiple isoforms. Although several studies have described the effect of GABA(A) receptor stimulation on LHRH and/or gonadotropin release in prepubertal animals, nothing is known about the receptor subunits that may be expressed in LHRH neurons at this phase in development. Double immunohistofluorescence followed by confocal laser microscopy revealed that subsets of prepubertal LHRH neurons are endowed with α1, α2, β(2/3), and γ2 GABA(A) receptor subunits. Combined immunohistochemistry for LHRH neurons and in situ hybridization for GABA(A) subunit mRNAs confirmed that the genes encoding the α1, α2, β3, and γ2 subnnits, but not the γ1 subunit, are expressed in LHRH neurons. Notwithstanding the relative insensitivity of these methods, both the immunohistochemical and hybridization histochemical approaches employed indicate that only a fraction of LHRH neurons are endowed with GABA(A) receptors. This arrangement suggests that those LHRH neurons bearing the appropriate GABA(A) receptors are responsible for either the entire secretory response to direct GABAergic inputs or for its initiation.

    KW - GABA(A) receptor

    KW - Hypothalamus

    KW - Immunohistochemistry

    KW - In situ hybridization

    KW - Sexual development

    UR - http://www.scopus.com/inward/record.url?scp=0032509663&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0032509663&partnerID=8YFLogxK

    U2 - 10.1016/S0006-8993(97)01152-9

    DO - 10.1016/S0006-8993(97)01152-9

    M3 - Article

    C2 - 9507141

    AN - SCOPUS:0032509663

    VL - 780

    SP - 218

    EP - 229

    JO - Brain Research

    JF - Brain Research

    SN - 0006-8993

    IS - 2

    ER -