Serotonin-related gene expression in female monkeys with individual sensitivity to stress

Cynthia Bethea, J. M. Streicher, S. J. Mirkes, R. L. Sanchez, Arubala Reddy, J. L. Cameron

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    Female cynomolgus monkeys exhibit different degrees of reproductive dysfunction with moderate metabolic and psychosocial stress. In this study, the expression of four genes pivotal to serotonin neural function was assessed in monkeys previously categorized as highly stress resistant (n=3; normal menstrual cyclicity through two stress cycles), medium stress resistant (n=5; ovulatory in the first stress cycle but anovulatory in the second stress cycle), or low stress resistant (i.e. stress-sensitive; n=4; anovulatory as soon as stress is initiated). In situ hybridization and quantitative image analysis was used to measure mRNAs coding for SERT (serotonin transporter), 5HT1A autoreceptor, MAO-A and MAO-B (monoamine oxidases) at six levels of the dorsal raphe nucleus (DRN). Optical density (OD) and positive pixel area were measured with NIH Image software. In addition, serotonin neurons were immunostained and counted at three levels of the DRN. Finally, each animal was genotyped for the serotonin transporter long polymorphic region (5HTTLPR). Stress sensitive animals had lower expression of SERT mRNA in the caudal region of the DRN (P

    Original languageEnglish (US)
    Pages (from-to)151-166
    Number of pages16
    JournalNeuroscience
    Volume132
    Issue number1
    DOIs
    StatePublished - 2005

    Fingerprint

    Serotonin Plasma Membrane Transport Proteins
    Monoamine Oxidase
    Haplorhini
    Serotonin
    Gene Expression
    Autoreceptors
    Messenger RNA
    Macaca fascicularis
    Periodicity
    In Situ Hybridization
    Software
    Neurons
    Dorsal Raphe Nucleus

    Keywords

    • Monoamine oxidase
    • Serotonin
    • Serotonin autoreceptor
    • Serotonin reuptake transporter
    • Steroids
    • Stress

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Bethea, C., Streicher, J. M., Mirkes, S. J., Sanchez, R. L., Reddy, A., & Cameron, J. L. (2005). Serotonin-related gene expression in female monkeys with individual sensitivity to stress. Neuroscience, 132(1), 151-166. https://doi.org/10.1016/j.neuroscience.2004.11.022

    Serotonin-related gene expression in female monkeys with individual sensitivity to stress. / Bethea, Cynthia; Streicher, J. M.; Mirkes, S. J.; Sanchez, R. L.; Reddy, Arubala; Cameron, J. L.

    In: Neuroscience, Vol. 132, No. 1, 2005, p. 151-166.

    Research output: Contribution to journalArticle

    Bethea, C, Streicher, JM, Mirkes, SJ, Sanchez, RL, Reddy, A & Cameron, JL 2005, 'Serotonin-related gene expression in female monkeys with individual sensitivity to stress', Neuroscience, vol. 132, no. 1, pp. 151-166. https://doi.org/10.1016/j.neuroscience.2004.11.022
    Bethea, Cynthia ; Streicher, J. M. ; Mirkes, S. J. ; Sanchez, R. L. ; Reddy, Arubala ; Cameron, J. L. / Serotonin-related gene expression in female monkeys with individual sensitivity to stress. In: Neuroscience. 2005 ; Vol. 132, No. 1. pp. 151-166.
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