Serotonin in microdialysate from the mediobasal hypothalamus increases after progesterone administration to estrogen primed macaques

Maria Luisa Centeno, Arubala Reddy, Lisa J. Smith, Rachel L. Sanchez, Jessica A. Henderson, Nurgul C. Salli, David Hess, Francis K Y Pau, Cynthia Bethea

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    Estrogen and progesterone act on gene and protein expression in serotonin neurons in a manner that suggests serotonin neurotransmission should increase. However, measurement of extracellular serotonin in macaques was lacking. Elevated prolactin secretion can be an indicator of increased serotonergic function and prolactin is increased by combined estrogen and progesterone treatment. We examined extracellular serotonin by microdialysis in a well-characterized macaque model of steroid-induced prolactin secretion. Monkeys were fitted with 2 guide tubes directed to the arcuate nucleus of the hypothalamus. Samples (75 μl/15-minute interval) were obtained via a tether-swivel device through sample lines into an adjoining room. Serotonin was measured with a modified commercial enzyme linked immunoassay (ELISA) kit. Fenfluramine infused through the probe (300 μM for 2 h; n = 2 trials) or administered intravenously (2.5 mg/kg; n = 2 trials) caused a marked increase in extracellular serotonin and verified the efficacy of the procedure. Three monkeys were maintained with an estrogen implant for 2 weeks. Each monkey was injected with 20 mg of progesterone s.c. in oil at 1500 h; microdialysis was initiated the next morning and samples were obtained for 24 h. There was a significant increase in serotonin between 40 and 43 h after the progesterone injection (P <0.001, ANOVA). Serotonin averaged 59 ± 1 pg/sample from 18-30 h post-progesterone injection, and averaged 76 ± 2 pg/sample from 30-48 h post-progesterone injection (P <0.0001; t-test). Since the increase in serotonin is delayed by ∼ 40 h after progesterone-injection, we speculate that the action of progesterone may involve either nuclear progestin receptors or membrane progestin receptors.

    Original languageEnglish (US)
    Pages (from-to)67-75
    Number of pages9
    JournalEuropean Journal of Pharmacology
    Volume555
    Issue number1
    DOIs
    StatePublished - Jan 19 2007

    Fingerprint

    Macaca
    Hypothalamus
    Progesterone
    Serotonin
    Estrogens
    Prolactin
    Haplorhini
    Injections
    Microdialysis
    Progesterone Receptors
    Fenfluramine
    Arcuate Nucleus of Hypothalamus
    Nuclear Envelope
    Cytoplasmic and Nuclear Receptors
    Immunoenzyme Techniques
    Synaptic Transmission
    Analysis of Variance
    Oils
    Steroids
    Gene Expression

    Keywords

    • Estrogen
    • Fenfluramine
    • Hypothalamus
    • Macaca mulatta
    • Microdialysis
    • Progesterone

    ASJC Scopus subject areas

    • Cellular and Molecular Neuroscience
    • Pharmacology

    Cite this

    Serotonin in microdialysate from the mediobasal hypothalamus increases after progesterone administration to estrogen primed macaques. / Centeno, Maria Luisa; Reddy, Arubala; Smith, Lisa J.; Sanchez, Rachel L.; Henderson, Jessica A.; Salli, Nurgul C.; Hess, David; Pau, Francis K Y; Bethea, Cynthia.

    In: European Journal of Pharmacology, Vol. 555, No. 1, 19.01.2007, p. 67-75.

    Research output: Contribution to journalArticle

    Centeno, Maria Luisa ; Reddy, Arubala ; Smith, Lisa J. ; Sanchez, Rachel L. ; Henderson, Jessica A. ; Salli, Nurgul C. ; Hess, David ; Pau, Francis K Y ; Bethea, Cynthia. / Serotonin in microdialysate from the mediobasal hypothalamus increases after progesterone administration to estrogen primed macaques. In: European Journal of Pharmacology. 2007 ; Vol. 555, No. 1. pp. 67-75.
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    abstract = "Estrogen and progesterone act on gene and protein expression in serotonin neurons in a manner that suggests serotonin neurotransmission should increase. However, measurement of extracellular serotonin in macaques was lacking. Elevated prolactin secretion can be an indicator of increased serotonergic function and prolactin is increased by combined estrogen and progesterone treatment. We examined extracellular serotonin by microdialysis in a well-characterized macaque model of steroid-induced prolactin secretion. Monkeys were fitted with 2 guide tubes directed to the arcuate nucleus of the hypothalamus. Samples (75 μl/15-minute interval) were obtained via a tether-swivel device through sample lines into an adjoining room. Serotonin was measured with a modified commercial enzyme linked immunoassay (ELISA) kit. Fenfluramine infused through the probe (300 μM for 2 h; n = 2 trials) or administered intravenously (2.5 mg/kg; n = 2 trials) caused a marked increase in extracellular serotonin and verified the efficacy of the procedure. Three monkeys were maintained with an estrogen implant for 2 weeks. Each monkey was injected with 20 mg of progesterone s.c. in oil at 1500 h; microdialysis was initiated the next morning and samples were obtained for 24 h. There was a significant increase in serotonin between 40 and 43 h after the progesterone injection (P <0.001, ANOVA). Serotonin averaged 59 ± 1 pg/sample from 18-30 h post-progesterone injection, and averaged 76 ± 2 pg/sample from 30-48 h post-progesterone injection (P <0.0001; t-test). Since the increase in serotonin is delayed by ∼ 40 h after progesterone-injection, we speculate that the action of progesterone may involve either nuclear progestin receptors or membrane progestin receptors.",
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    AU - Sanchez, Rachel L.

    AU - Henderson, Jessica A.

    AU - Salli, Nurgul C.

    AU - Hess, David

    AU - Pau, Francis K Y

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