Serotonergic lesioning differentially affects presynaptic and postsynaptic 5-HT1B receptor mRNA levels in rat brain

John F. Neumaier, Patricia Szot, Elaine R. Peskind, Daniel Dorsa, Mark W. Hamblin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The rat 5-HT1B receptor (also referred to as the 5-HT1Dβ receptor) is expressed in both serotonergic and nonserotonergic neurons in the rat brain, where it has been hypothesized to inhibit the release of neurotransmitters from axonal terminals. In this study we investigated the effect of chemical axotomy of serotonergic processes by 5,7-dihydroxytryptamine on the levels of 5-HT1B mRNA in the dorsal raphe nucleus and several postsynaptic brain areas using in situ hybridization. 5,7-Dihydroxytryptamine (i.c.v.) reduced forebrain [3H]citalopram binding to serotonin transporter by 62-96% whereas binding in the dorsal raphe nucleus was preserved. Serotonin transporter mRNA hybridization signal in the dorsal raphe nucleus was only slightly reduced after 5,7-dihydroxytryptamine. These results suggest that our lesioning protocol caused axonal degeneration with preservation of most of the serotonergic perikarya in the dorsal raphe nucleus. 5-HT1B mRNA hybridization signal in postsynaptic regions was unchanged by serotonergic lesions, but was markedly reduced in the dorsal raphe nucleus. Thus, disruption of serotonergic innervation affects the regulation of presynaptic and postsynaptic 5-HT1B mRNA differently. Furthermore, although both 5-HT1B receptors and serotonin transporters are found in serotonergic terminals, their levels may be regulated differentially during the period of regrowth that follows chemical axotomy.

Original languageEnglish (US)
Pages (from-to)50-58
Number of pages9
JournalBrain Research
Volume722
Issue number1-2
StatePublished - May 25 1996
Externally publishedYes

Fingerprint

Receptor, Serotonin, 5-HT1B
5,7-Dihydroxytryptamine
Serotonin Plasma Membrane Transport Proteins
Messenger RNA
Brain
Axotomy
Receptor, Serotonin, 5-HT1D
Serotonergic Neurons
Citalopram
Prosencephalon
In Situ Hybridization
Neurotransmitter Agents
Dorsal Raphe Nucleus

Keywords

  • 5,7-dihydroxytryptamine
  • 5-HT receptor
  • 5-HT receptor
  • Autoreceptor
  • Citalopram
  • Dorsal raphe
  • Serotonin
  • Serotonin receptor
  • Serotonin transporter

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Serotonergic lesioning differentially affects presynaptic and postsynaptic 5-HT1B receptor mRNA levels in rat brain. / Neumaier, John F.; Szot, Patricia; Peskind, Elaine R.; Dorsa, Daniel; Hamblin, Mark W.

In: Brain Research, Vol. 722, No. 1-2, 25.05.1996, p. 50-58.

Research output: Contribution to journalArticle

Neumaier, John F. ; Szot, Patricia ; Peskind, Elaine R. ; Dorsa, Daniel ; Hamblin, Mark W. / Serotonergic lesioning differentially affects presynaptic and postsynaptic 5-HT1B receptor mRNA levels in rat brain. In: Brain Research. 1996 ; Vol. 722, No. 1-2. pp. 50-58.
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