Serological visualization of interleukin 2

Gerhard Steinmann, Paul Conlon, Steven Hefeneider, Steven Gillis

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Interleukin 2, a lymphokine that acts as a second signal of cellular immune response by way of its action as a T-cell growth factor, was morphologically identified by immunoperoxidase staining. With the use of a monoclonal antibody to interleukin 2 and several complex-forming antisera, the lymphokine was readily distinguished in cytocentrifuge preparations of peripheral blood leukocytes stimulated with a T-cell mitogen. When preparations of cloned interleukin 2 producer and responder cells were stained by the same procedures, discrete patterns of both responder and producer cell phenotypes were revealed. Interleukin 2 producer T cells exhibited a characteristic intense, ringlike cytoplasmic staining, whereas the responder cells (as exemplified by interleukin 2-dependent cell lines) exhibited a less intensive, spotlike membrane staining. In addition, intense membrane localization of interleukin 2, reminiscent of potential capping phenomena, could be observed in stained preparations of cloned responder cells.

Original languageEnglish (US)
Pages (from-to)1188-1190
Number of pages3
JournalScience
Volume220
Issue number4602
DOIs
StatePublished - Jan 1 1983

ASJC Scopus subject areas

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    Steinmann, G., Conlon, P., Hefeneider, S., & Gillis, S. (1983). Serological visualization of interleukin 2. Science, 220(4602), 1188-1190. https://doi.org/10.1126/science.6344215