Serial dilution curve: A new method for analysis of reverse phase protein array data

Li Zhang, Qingyi Wei, Li Mao, Wenbin Liu, Gordon Mills, Kevin Coombes

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there is usually no known protein concentration from which one can construct a standard response curve. Here, we describe a new method called 'serial dilution curve for RPPA data analysis'. Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations.

Original languageEnglish (US)
Pages (from-to)650-654
Number of pages5
JournalBioinformatics
Volume25
Issue number5
DOIs
StatePublished - Mar 1 2009
Externally publishedYes

Fingerprint

Protein Array Analysis
Dilution
Reverse
Proteins
Protein
Curve
Enzyme-Linked Immunosorbent Assay
Enzyme-linked Immunosorbent Assay
Technology
Data Quality
Dynamic Range
Quantification
High Throughput
Confidence
Assays
Data analysis
Throughput
Series
Experiment

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computational Theory and Mathematics
  • Computer Science Applications
  • Computational Mathematics
  • Statistics and Probability

Cite this

Serial dilution curve : A new method for analysis of reverse phase protein array data. / Zhang, Li; Wei, Qingyi; Mao, Li; Liu, Wenbin; Mills, Gordon; Coombes, Kevin.

In: Bioinformatics, Vol. 25, No. 5, 01.03.2009, p. 650-654.

Research output: Contribution to journalArticle

Zhang, Li ; Wei, Qingyi ; Mao, Li ; Liu, Wenbin ; Mills, Gordon ; Coombes, Kevin. / Serial dilution curve : A new method for analysis of reverse phase protein array data. In: Bioinformatics. 2009 ; Vol. 25, No. 5. pp. 650-654.
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