Sequence, linkage to H2-K,and function of mouse tapasin in MHC class I assembly

Andres G. Grandea, Paul G. Comber, Scott E. Wenderfer, Gary Schoenhals, Klaus Früh, John J. Monaco, Thomas Spies

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Assembly of major histocompatibility complex (MHC) class I molecules in human cells is dependent on the accessory protein tapasin, which mediates their interaction with the transporters associated with antigen processing (TAP) and thereby ensures efficient peptide binding. Analysis of a mouse tapasin complementary DNA defined a conserved polypeptide sharing sequences diagnostic of a transmembrane protein related to the immunoglobulin superfamily, and an endoplasmic reticulum retention motif. The mouse tapasin gene was mapped about 70 kilobases from H2-K at the centromeric end of the mouse MHC. Expression of mouse tapasin in a tapasin-deficient human mutant cell line restored the normal assembly and expression of class I alleles. Thus, tapasin is a structurally and functionally conserved component of the MHC class I antigen processing pathway. Its genetic linkage to the class I and TAP subunit genes in the MHC may be of significance in the coordinate expression and functional coadaptation of the diverse gene products.

Original languageEnglish (US)
Pages (from-to)260-265
Number of pages6
JournalImmunogenetics
Volume48
Issue number4
DOIs
StatePublished - Sep 10 1998

    Fingerprint

Keywords

  • Assembly
  • MHC class I
  • TAP
  • Tapasin

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Grandea, A. G., Comber, P. G., Wenderfer, S. E., Schoenhals, G., Früh, K., Monaco, J. J., & Spies, T. (1998). Sequence, linkage to H2-K,and function of mouse tapasin in MHC class I assembly. Immunogenetics, 48(4), 260-265. https://doi.org/10.1007/s002510050430