Separation of μ-opioid receptor desensitization and internalization: Endogenous receptors in primary neuronal cultures

Seksiri Arttamangkul, Maria Torrecilla, Kazuto Kobayashi, Hideyuki Okano, John Williams

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

A close relationship between desensitization and internalization of μ-opioid receptors (MORs) has been proposed based on differential actions of series of agonists. The role that these two processes have in the development of tolerance and dependence to opioids has been a controversial subject that has been studied in a variety of model systems. Here, we examine desensitization and internalization of endogenous MORs simultaneously in primary cultures of locus ceruleus neurons using fluorescently tagged peptide agonists. With the use of two fluorescent opioid peptides, dermorphin-Bodipy Texas Red and dermorphin-Alexa594 (Derm-A594), desensitization was measured electrophysiologically and trafficking was followed by the accumulation of intracellular fluorescent puncta. Blocking endocytosis with concanavalin A eliminated the accumulation of fluorescent puncta but desensitization induced by Derm-A594 was unaffected. Likewise, after treatment with concanavalin A, there was no change in either desensitization or recovery from desensitization induced by [Met]5 enkephalin. The results demonstrate that desensitization and the recovery from desensitization are not dependent on receptor internalization and suggest that the activity of endogenous MORs in primary neurons can be modulated at the level of the plasma membrane.

Original languageEnglish (US)
Pages (from-to)4118-4125
Number of pages8
JournalJournal of Neuroscience
Volume26
Issue number15
DOIs
StatePublished - 2006

Fingerprint

Opioid Receptors
Concanavalin A
Neurons
Methionine Enkephalin
Locus Coeruleus
Opioid Peptides
Endocytosis
Opioid Analgesics
Cell Membrane
Peptides
dermorphin
Alexa594

Keywords

  • Concanavalin A
  • Culture
  • Electrophysiology
  • Fluorescent-peptide
  • Locus ceruleus
  • Two-photon microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Separation of μ-opioid receptor desensitization and internalization : Endogenous receptors in primary neuronal cultures. / Arttamangkul, Seksiri; Torrecilla, Maria; Kobayashi, Kazuto; Okano, Hideyuki; Williams, John.

In: Journal of Neuroscience, Vol. 26, No. 15, 2006, p. 4118-4125.

Research output: Contribution to journalArticle

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