Sensorless adaptive optics multimodal en-face small animal retinal imaging

Daniel J. Wahl, Ringo Ng, Myeong Jin Ju, Yifan Jian, Marinko V. Sarunic

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Vision researchers often use small animals due to the availability of many transgenic strains that model human diseases or express biomarkers. Adaptive optics (AO) enables non-invasive single-cell imaging in a living animal but often results in high system complexity. Sensorless AO (SAO) can provide depth-resolved aberration correction with low system complexity. We present a multi-modal sensorless AO en face retina imaging system that includes optical coherence tomography (OCT), OCT-angiography, confocal scanning laser ophthalmoscopy (SLO), and fluorescence detection. We present a compact lens-based imaging system design that allows for a 50-degree maximum field of view (FOV), which can be reduced to the region of interest to perform SAO with the modality of choice. The system performance was demonstrated on wild type mice (C57BL/6J), and transgenic mice with GFP labeled cells. SAO SLO was used for imaging microglia (Cx3cr1-GFP) over ~1 hour, where dynamics of the microglia branches were clearly observed. Our results also include volumetric cellular imaging of microglia throughout the inner retina.

Original languageEnglish (US)
Pages (from-to)252-266
Number of pages15
JournalBiomedical Optics Express
Volume10
Issue number1
DOIs
StatePublished - 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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