TY - JOUR
T1 - Sensor array
T2 - Impedimetric label-free sensing of DNA hybridization in real time for rapid, PCR-based detection of microorganisms
AU - Ghindilis, Andrei L.
AU - Smith, Maria W.
AU - Schwarzkopf, Kevin R.
AU - Zhan, Changqing
AU - Evans, David R.
AU - Baptista, António M.
AU - Simon, Holly M.
PY - 2009/7
Y1 - 2009/7
N2 - A novel, impedance-based electronic sensor format was used for label-free, real-time detection of microbial DNA on oligonucleotide probe arrays. Detection limits of 5 - 10 nM were achieved for single-stranded, PCR-amplified DNA targets. Hybridization selectivity yielded 9- to 12-fold signal increases for specific targets, and the sensor arrays were re-used multiple times without significant signal degradation. These and other features of the SHARP Laboratories of America (SLA) sensor array, such as its ability to acquire continuous measurements of DNA as it accumulates on the array surface, make it an attractive biosensor platform for field detection and monitoring of sentinel and/or pathogenic microorganisms.
AB - A novel, impedance-based electronic sensor format was used for label-free, real-time detection of microbial DNA on oligonucleotide probe arrays. Detection limits of 5 - 10 nM were achieved for single-stranded, PCR-amplified DNA targets. Hybridization selectivity yielded 9- to 12-fold signal increases for specific targets, and the sensor arrays were re-used multiple times without significant signal degradation. These and other features of the SHARP Laboratories of America (SLA) sensor array, such as its ability to acquire continuous measurements of DNA as it accumulates on the array surface, make it an attractive biosensor platform for field detection and monitoring of sentinel and/or pathogenic microorganisms.
KW - DNA
KW - Environmental chemistry
KW - Impedance sensor array
KW - Microorganism detection
KW - Polymerase chain reaction
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U2 - 10.1002/elan.200904549
DO - 10.1002/elan.200904549
M3 - Article
AN - SCOPUS:69249230990
SN - 1040-0397
VL - 21
SP - 1459
EP - 1468
JO - Electroanalysis
JF - Electroanalysis
IS - 13
ER -