Sensor array: Impedimetric label-free sensing of DNA hybridization in real time for rapid, PCR-based detection of microorganisms

Andrei L. Ghindilis, Maria W. Smith, Kevin R. Schwarzkopf, Changqing Zhan, David R. Evans, Antonio Baptista, Holly Simon

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A novel, impedance-based electronic sensor format was used for label-free, real-time detection of microbial DNA on oligonucleotide probe arrays. Detection limits of 5 - 10 nM were achieved for single-stranded, PCR-amplified DNA targets. Hybridization selectivity yielded 9- to 12-fold signal increases for specific targets, and the sensor arrays were re-used multiple times without significant signal degradation. These and other features of the SHARP Laboratories of America (SLA) sensor array, such as its ability to acquire continuous measurements of DNA as it accumulates on the array surface, make it an attractive biosensor platform for field detection and monitoring of sentinel and/or pathogenic microorganisms.

Original languageEnglish (US)
Pages (from-to)1459-1468
Number of pages10
JournalElectroanalysis
Volume21
Issue number13
DOIs
StatePublished - Jul 2009

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Sensor arrays
Microorganisms
Labels
DNA
Oligonucleotide Probes
Oligonucleotides
Biosensors
Degradation
Monitoring
Sensors

Keywords

  • DNA
  • Environmental chemistry
  • Impedance sensor array
  • Microorganism detection
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Sensor array : Impedimetric label-free sensing of DNA hybridization in real time for rapid, PCR-based detection of microorganisms. / Ghindilis, Andrei L.; Smith, Maria W.; Schwarzkopf, Kevin R.; Zhan, Changqing; Evans, David R.; Baptista, Antonio; Simon, Holly.

In: Electroanalysis, Vol. 21, No. 13, 07.2009, p. 1459-1468.

Research output: Contribution to journalArticle

Ghindilis, Andrei L. ; Smith, Maria W. ; Schwarzkopf, Kevin R. ; Zhan, Changqing ; Evans, David R. ; Baptista, Antonio ; Simon, Holly. / Sensor array : Impedimetric label-free sensing of DNA hybridization in real time for rapid, PCR-based detection of microorganisms. In: Electroanalysis. 2009 ; Vol. 21, No. 13. pp. 1459-1468.
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