Sensitivity and specificity of cerebrospinal fluid flow cytometry for the diagnosis of leukemic meningitis in acute lymphoblastic leukemia/lymphoma

Zahi Mitri, Momin T. Siddiqui, Fuad El Rassi, Jeannine T. Holden, Leonard T. Heffner, Amelia Langston, Edmund K. Waller, Elliott Winton, Morgan McLemore, Leon Bernal-Mizrachi, David Jaye, Martha Arellano, Hanna Jean Khoury

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The presence of leukemic blasts detected by light microscopy in cerebrospinal fluid (CSF) establishes the diagnosis of leukemic meningitis in acute lymphoblastic leukemia/lymphoma (ALL). Flow cytometry immunophenotyping (FCI) is a very sensitive method that detects a minute number of aberrant cells, and is increasingly performed on CSF samples. We sought to determine the sensitivity and specificity of CSF FCI for the diagnosis of leukemic meningitis in ALL. Between November 2007 and August 2011, 800 CSF samples from 80 patients with ALL were available from diagnostic lumbar punctures (LPs; n = 80), follow-up LPs (n = 687) and at the time of relapse (n = 33). FCI was performed on 267 samples, and only identified aberrant cells in cytologically confirmed cases of leukemic meningitis. A blinded review of all cases with detectable CSF nucleated cells confirmed these findings. We conclude that CSF FCI has a 100% sensitivity and specificity for the detection of lymphoblasts. However, additional studies are needed to define the role this procedure plays in the diagnosis of leukemic meningitis.

Original languageEnglish (US)
Pages (from-to)1498-1500
Number of pages3
JournalLeukemia and Lymphoma
Volume55
Issue number7
DOIs
StatePublished - Jul 2014

Keywords

  • Cerebrospinal fluid
  • Flow cytometry
  • Lymphoblastic lymphoma
  • Sensitivity
  • Specificity

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Mitri, Z., Siddiqui, M. T., El Rassi, F., Holden, J. T., Heffner, L. T., Langston, A., Waller, E. K., Winton, E., McLemore, M., Bernal-Mizrachi, L., Jaye, D., Arellano, M., & Khoury, H. J. (2014). Sensitivity and specificity of cerebrospinal fluid flow cytometry for the diagnosis of leukemic meningitis in acute lymphoblastic leukemia/lymphoma. Leukemia and Lymphoma, 55(7), 1498-1500. https://doi.org/10.3109/10428194.2013.852667