Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus: Role of nerve growth factor as an ovulation-inducing factor

Marco A. Berland, Cesar Ulloa-Leal, Miguel Barría, Hollis Wright, Gregory Dissen, Mauricio E. Silva, Sergio Ojeda, Marcelo H. Ratto

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in semen instead of being mediated by neural signals. The present study examines this notion. Llamas displaying a preovulatory follicle were assigned to four groups: group 1 received an intrauterine infusion (IUI) of PBS; group 2 received an IUI of seminal plasma; group 3 was mated to a male whose urethra had been surgically diverted (urethrostomized male); and group 4 was mated to an intact male. Ovulation (detected by ultrasonography) occurred only in llamas mated to an intact male or given an IUI of seminal plasma and was preceded by a surge in plasma LH levels initiated within an hour after coitus or IUI. In both ovulatory groups, circulating β-NGF levels increased within 15 minutes after treatment, reaching values that were greater and more sustained in llamas mated with an intact male. These results demonstrate that llamas can be induced to ovulate by seminal plasma in the absence of copulation and that copulation alone cannot elicit ovulation in the absence of seminal plasma. In addition, our results implicate β-NGF as an important mediator of seminal plasma-induced ovulation in llamas because ovulation does not occur if β-NGF levels do not increase in the bloodstream, a change that occurs promptly after copulation with an intact male or IUI of seminal plasma.

    Original languageEnglish (US)
    Pages (from-to)3224-3232
    Number of pages9
    JournalEndocrinology
    Volume157
    Issue number8
    DOIs
    StatePublished - Aug 1 2016

    Fingerprint

    New World Camelids
    Nerve Growth Factor
    Ovulation
    Semen
    Copulation
    Coitus
    Urethra
    Reflex
    Ultrasonography

    ASJC Scopus subject areas

    • Medicine(all)
    • Endocrinology

    Cite this

    Berland, M. A., Ulloa-Leal, C., Barría, M., Wright, H., Dissen, G., Silva, M. E., ... Ratto, M. H. (2016). Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus: Role of nerve growth factor as an ovulation-inducing factor. Endocrinology, 157(8), 3224-3232. https://doi.org/10.1210/en.2016-1310

    Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus : Role of nerve growth factor as an ovulation-inducing factor. / Berland, Marco A.; Ulloa-Leal, Cesar; Barría, Miguel; Wright, Hollis; Dissen, Gregory; Silva, Mauricio E.; Ojeda, Sergio; Ratto, Marcelo H.

    In: Endocrinology, Vol. 157, No. 8, 01.08.2016, p. 3224-3232.

    Research output: Contribution to journalArticle

    Berland, Marco A. ; Ulloa-Leal, Cesar ; Barría, Miguel ; Wright, Hollis ; Dissen, Gregory ; Silva, Mauricio E. ; Ojeda, Sergio ; Ratto, Marcelo H. / Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus : Role of nerve growth factor as an ovulation-inducing factor. In: Endocrinology. 2016 ; Vol. 157, No. 8. pp. 3224-3232.
    @article{b25d3abce624475d8fea1dbe1e8a1316,
    title = "Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus: Role of nerve growth factor as an ovulation-inducing factor",
    abstract = "Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in semen instead of being mediated by neural signals. The present study examines this notion. Llamas displaying a preovulatory follicle were assigned to four groups: group 1 received an intrauterine infusion (IUI) of PBS; group 2 received an IUI of seminal plasma; group 3 was mated to a male whose urethra had been surgically diverted (urethrostomized male); and group 4 was mated to an intact male. Ovulation (detected by ultrasonography) occurred only in llamas mated to an intact male or given an IUI of seminal plasma and was preceded by a surge in plasma LH levels initiated within an hour after coitus or IUI. In both ovulatory groups, circulating β-NGF levels increased within 15 minutes after treatment, reaching values that were greater and more sustained in llamas mated with an intact male. These results demonstrate that llamas can be induced to ovulate by seminal plasma in the absence of copulation and that copulation alone cannot elicit ovulation in the absence of seminal plasma. In addition, our results implicate β-NGF as an important mediator of seminal plasma-induced ovulation in llamas because ovulation does not occur if β-NGF levels do not increase in the bloodstream, a change that occurs promptly after copulation with an intact male or IUI of seminal plasma.",
    author = "Berland, {Marco A.} and Cesar Ulloa-Leal and Miguel Barr{\'i}a and Hollis Wright and Gregory Dissen and Silva, {Mauricio E.} and Sergio Ojeda and Ratto, {Marcelo H.}",
    year = "2016",
    month = "8",
    day = "1",
    doi = "10.1210/en.2016-1310",
    language = "English (US)",
    volume = "157",
    pages = "3224--3232",
    journal = "Endocrinology",
    issn = "0013-7227",
    publisher = "The Endocrine Society",
    number = "8",

    }

    TY - JOUR

    T1 - Seminal plasma induces ovulation in llamas in the absence of a copulatory stimulus

    T2 - Role of nerve growth factor as an ovulation-inducing factor

    AU - Berland, Marco A.

    AU - Ulloa-Leal, Cesar

    AU - Barría, Miguel

    AU - Wright, Hollis

    AU - Dissen, Gregory

    AU - Silva, Mauricio E.

    AU - Ojeda, Sergio

    AU - Ratto, Marcelo H.

    PY - 2016/8/1

    Y1 - 2016/8/1

    N2 - Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in semen instead of being mediated by neural signals. The present study examines this notion. Llamas displaying a preovulatory follicle were assigned to four groups: group 1 received an intrauterine infusion (IUI) of PBS; group 2 received an IUI of seminal plasma; group 3 was mated to a male whose urethra had been surgically diverted (urethrostomized male); and group 4 was mated to an intact male. Ovulation (detected by ultrasonography) occurred only in llamas mated to an intact male or given an IUI of seminal plasma and was preceded by a surge in plasma LH levels initiated within an hour after coitus or IUI. In both ovulatory groups, circulating β-NGF levels increased within 15 minutes after treatment, reaching values that were greater and more sustained in llamas mated with an intact male. These results demonstrate that llamas can be induced to ovulate by seminal plasma in the absence of copulation and that copulation alone cannot elicit ovulation in the absence of seminal plasma. In addition, our results implicate β-NGF as an important mediator of seminal plasma-induced ovulation in llamas because ovulation does not occur if β-NGF levels do not increase in the bloodstream, a change that occurs promptly after copulation with an intact male or IUI of seminal plasma.

    AB - Llamas are considered to be reflex ovulators. However, semen from these animals is reported to be rich in ovulation-inducing factor(s), one of which has been identified as nerve growth factor (NGF). These findings suggest that ovulation in llamas may be elicited by chemical signals contained in semen instead of being mediated by neural signals. The present study examines this notion. Llamas displaying a preovulatory follicle were assigned to four groups: group 1 received an intrauterine infusion (IUI) of PBS; group 2 received an IUI of seminal plasma; group 3 was mated to a male whose urethra had been surgically diverted (urethrostomized male); and group 4 was mated to an intact male. Ovulation (detected by ultrasonography) occurred only in llamas mated to an intact male or given an IUI of seminal plasma and was preceded by a surge in plasma LH levels initiated within an hour after coitus or IUI. In both ovulatory groups, circulating β-NGF levels increased within 15 minutes after treatment, reaching values that were greater and more sustained in llamas mated with an intact male. These results demonstrate that llamas can be induced to ovulate by seminal plasma in the absence of copulation and that copulation alone cannot elicit ovulation in the absence of seminal plasma. In addition, our results implicate β-NGF as an important mediator of seminal plasma-induced ovulation in llamas because ovulation does not occur if β-NGF levels do not increase in the bloodstream, a change that occurs promptly after copulation with an intact male or IUI of seminal plasma.

    UR - http://www.scopus.com/inward/record.url?scp=84982860178&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84982860178&partnerID=8YFLogxK

    U2 - 10.1210/en.2016-1310

    DO - 10.1210/en.2016-1310

    M3 - Article

    C2 - 27355492

    AN - SCOPUS:84982860178

    VL - 157

    SP - 3224

    EP - 3232

    JO - Endocrinology

    JF - Endocrinology

    SN - 0013-7227

    IS - 8

    ER -