Self-assembled cellular microarrays patterned using DNA barcodes

Erik S. Douglas, Ravi A. Chandra, Carolyn R. Bertozzi, Richard A. Mathies, Matthew B. Francis

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

The successful integration of living cells into synthetic devices requires precise control over cell patterning. Here we describe a versatile platform that can accomplish this goal through DNA hybridization. Living cells functionalized with exogenous cell-surface DNA strands bind to cognate sequences of DNA printed on glass slides. Attachment via these "cell-adhesion barcodes" is rapid and specific, with close-packed arrays of cells forming within minutes. The biophysical properties of the system are characterized, and the technique is used to form complex cellular patterns with single-cell line widths and self-assembled cellular microarrays. Key advantages of DNA-directed cell binding include the ability to immobilize both adherent and non-adherent cells, to capture cells selectively from a mixed population, to tune the binding properties of the cells, and to reuse substrates prepared with widely available DNA printing technologies.

Original languageEnglish (US)
Pages (from-to)1442-1448
Number of pages7
JournalLab on a Chip
Volume7
Issue number11
DOIs
StatePublished - 2007
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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