Selective vasodilator effects of atrial natriuretic peptide in the human placental vasculature

Gershon Holcberg, Wilhelm Kossenjans, Anthony Brewer, Menachem Miodovnik, Leslie Myatt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective:To determine whether atrial natriuretic peptide (ANP) attenuates the vasoconstrictor effects of angiotensin II (AII), a thromboxane mimetic (U46619), and endothelin-1 in the human fetal-placental vasculature and to determine whether nitric oxide (NO) has a role in the vasodilator activity of ANP. Methods:Isolated placental cotyledons were dually perfused, with fetal perfusion pressure used as an index of vascular response. The effects of All (10-10-10-6 mol/L bolus injection), endothelin-1 (10-7 mol/L bolus), and U46619 (10-9-10-6 mol/L bolus or 10-8 mol/L infusion) were established in the absence or presence of ANP 10-8 mol/L). The role of NO as a mediator of ANP action was investigated by perfusion with n-nitro-L-arginine (NNLA, 10-3 mol/L), an inhibitor of NO synthase. Statistical significance was determined by analysis of variance. Results:Atrial natriuretic peptide caused significant attenuation of vasoconstrictor responses to AII, but weak attenuation of endothelin-1 and no attenuation of U46619. Use of NNLA did not affect the attenuation of AII-induced vasoconstriction by ANP. Conclusion:Atrial natriuretic peptide is a vasodilator of the fetal-placental vasculature constricted with AII and endothelin-1, but not with U46619. Nitric oxide does not mediate the action of ANP.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of the Society for Gynecologic Investigation
Volume2
Issue number1
StatePublished - Jan 1995
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Vasodilator Agents
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Endothelin-1
Angiotensin II
Nitric Oxide
Vasoconstrictor Agents
Perfusion
Cotyledon
Thromboxanes
Vasoconstriction
Nitric Oxide Synthase
Blood Vessels
Arginine
Analysis of Variance
Pressure
Injections

Keywords

  • angiotensin
  • Atrial natriuretic peptide
  • endothelin-1
  • nitric oxide
  • placenta
  • thromboxane mimetic

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Selective vasodilator effects of atrial natriuretic peptide in the human placental vasculature. / Holcberg, Gershon; Kossenjans, Wilhelm; Brewer, Anthony; Miodovnik, Menachem; Myatt, Leslie.

In: Journal of the Society for Gynecologic Investigation, Vol. 2, No. 1, 01.1995, p. 1-5.

Research output: Contribution to journalArticle

Holcberg, Gershon ; Kossenjans, Wilhelm ; Brewer, Anthony ; Miodovnik, Menachem ; Myatt, Leslie. / Selective vasodilator effects of atrial natriuretic peptide in the human placental vasculature. In: Journal of the Society for Gynecologic Investigation. 1995 ; Vol. 2, No. 1. pp. 1-5.
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