TY - JOUR
T1 - Selective neurohormonal interactions in islet cell secretion in the isolated perfused human pancreas
AU - Brunicardi, F. Charles
AU - Druck, Paul
AU - Seymour, Neal E.
AU - Sun, You Su
AU - Elahi, Dariush
AU - Andersen, Dana K.
N1 - Funding Information:
The studies were supported by the Foundation for Surgical Education and Investigation, Inc., Brooklyn, New York, and the National Institutes of Health, Bethesda, Maryland (Grant AM-30336). The authors acknowledge the invaluable contribution of the Transplantation Service of the Department of Surgery, SUNY-HSC, and in particular the assistance of Mr. William Canterino. The technical assistance of Mr. Edward Rademaker is appreciated, and the authors acknowledge the assistance of Ms. Rosa Rivera in the preparation of the manuscript.
PY - 1990/4
Y1 - 1990/4
N2 - To investigate the effects of stimulant interactions on α- and β-cell secretions, the differential effects of gastric inhibitory polypeptide (GIP) and cholinergic stimulation (CS) on insulin (IRI) and glucagon (IRG) release were examined during euglycemic, single-pass perfusion in the isolated human pancreas. Pancreata obtained from 12 cadaver organ donors were perfused for 15-min test periods with (a) 1 nM GIP (b) intrinsic CS via bipolar electrical stimulation (10 V, 5 msec, 10 Hz) of the splanchnic neural fibers during simultaneous perfusion with 4 μM phentolamine and 6 μM propranolol, or (c) GIP and CS. The integrated response of IRI and IRG demonstrated that IRI release was stimulated 308 ± 52 μU/g-min by GIP, 366 ± 73 μU/g-min by CS, and 560 ± 50 μU/g-min by GIP and CS (P < 0.05). IRG release was stimulated 111 ± 33 pg/g-min by GIP, 34 ± 12 pg/g-min by CS, and 90 ± 36 pg/g-min by GIP and CS. Combined hormonal and cholinergic stimulation was additive for IRI release, but not for IRG release. We conclude that the interaction of neural and hormonal islet cell stimuli is cell-type specific. This may result in selective impairment of hormone release after pancreatic denervation.
AB - To investigate the effects of stimulant interactions on α- and β-cell secretions, the differential effects of gastric inhibitory polypeptide (GIP) and cholinergic stimulation (CS) on insulin (IRI) and glucagon (IRG) release were examined during euglycemic, single-pass perfusion in the isolated human pancreas. Pancreata obtained from 12 cadaver organ donors were perfused for 15-min test periods with (a) 1 nM GIP (b) intrinsic CS via bipolar electrical stimulation (10 V, 5 msec, 10 Hz) of the splanchnic neural fibers during simultaneous perfusion with 4 μM phentolamine and 6 μM propranolol, or (c) GIP and CS. The integrated response of IRI and IRG demonstrated that IRI release was stimulated 308 ± 52 μU/g-min by GIP, 366 ± 73 μU/g-min by CS, and 560 ± 50 μU/g-min by GIP and CS (P < 0.05). IRG release was stimulated 111 ± 33 pg/g-min by GIP, 34 ± 12 pg/g-min by CS, and 90 ± 36 pg/g-min by GIP and CS. Combined hormonal and cholinergic stimulation was additive for IRI release, but not for IRG release. We conclude that the interaction of neural and hormonal islet cell stimuli is cell-type specific. This may result in selective impairment of hormone release after pancreatic denervation.
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U2 - 10.1016/0022-4804(90)90058-A
DO - 10.1016/0022-4804(90)90058-A
M3 - Article
C2 - 1971030
AN - SCOPUS:0025303290
SN - 0022-4804
VL - 48
SP - 273
EP - 278
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 4
ER -