TY - JOUR
T1 - Selective modification and purification of phosphopeptides for automated sequence analysis
AU - Soderling, Thomas R.
AU - Walsh, Kenneth
N1 - Funding Information:
This work was supported by Grants AM National Instituteso f Health.
PY - 1982
Y1 - 1982
N2 - A method is described for the selective purification of 32P-labeled peptides for sequence analysis. The procedure is based on the differential retention on reversed-phase high-performance liquid chromatography of the peptide containing phosphoserine versus dehydroalanine obtained by alkaline β-elimination of the phosphate. The method was tested using two synthetic phosphopeptides representing the phosphorylation site in pyruvate kinase and one of the sites in glycogen synthase.
AB - A method is described for the selective purification of 32P-labeled peptides for sequence analysis. The procedure is based on the differential retention on reversed-phase high-performance liquid chromatography of the peptide containing phosphoserine versus dehydroalanine obtained by alkaline β-elimination of the phosphate. The method was tested using two synthetic phosphopeptides representing the phosphorylation site in pyruvate kinase and one of the sites in glycogen synthase.
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U2 - 10.1016/S0021-9673(01)88382-5
DO - 10.1016/S0021-9673(01)88382-5
M3 - Article
AN - SCOPUS:49049133041
SN - 0021-9673
VL - 253
SP - 243
EP - 251
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - C
ER -