Selective modification and purification of phosphopeptides for automated sequence analysis

Thomas Soderling, Kenneth Walsh

Research output: Contribution to journalArticle

12 Scopus citations


A method is described for the selective purification of 32P-labeled peptides for sequence analysis. The procedure is based on the differential retention on reversed-phase high-performance liquid chromatography of the peptide containing phosphoserine versus dehydroalanine obtained by alkaline β-elimination of the phosphate. The method was tested using two synthetic phosphopeptides representing the phosphorylation site in pyruvate kinase and one of the sites in glycogen synthase.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalJournal of Chromatography A
Issue numberC
Publication statusPublished - 1982
Externally publishedYes


ASJC Scopus subject areas

  • Analytical Chemistry

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