Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Lai Qiang Ying, Bruce Branchaud

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.

Original languageEnglish (US)
Pages (from-to)3546-3549
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume21
Issue number12
DOIs
StatePublished - Jun 15 2011
Externally publishedYes

Fingerprint

pH sensors
Lysosomes
Labeling
Cells
Flow cytometry
Monitoring
Fluorescence microscopy
Physiological Phenomena
Labels
Pathologic Processes
Fluorescence Microscopy
Wavelength
Flow Cytometry
4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

Keywords

  • Cell imaging
  • Confocal microscopy
  • Fluorescent probe
  • Lysosome
  • pH sensor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors. / Ying, Lai Qiang; Branchaud, Bruce.

In: Bioorganic and Medicinal Chemistry Letters, Vol. 21, No. 12, 15.06.2011, p. 3546-3549.

Research output: Contribution to journalArticle

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