Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Lai Qiang Ying; Bruce P. Branchaud

doi:10.1016/j.bmcl.2011.04.137

Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Lai Qiang Ying, Bruce P. Branchaud

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.

Original language	English (US)
Pages (from-to)	3546-3549
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	21
Issue number	12
DOIs	https://doi.org/10.1016/j.bmcl.2011.04.137
State	Published - Jun 15 2011
Externally published	Yes

Keywords

Cell imaging
Confocal microscopy
Fluorescent probe
Lysosome
pH sensor

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2011.04.137

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title = "Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors",

abstract = "New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.",

keywords = "Cell imaging, Confocal microscopy, Fluorescent probe, Lysosome, pH sensor",

author = "Ying, {Lai Qiang} and Branchaud, {Bruce P.}",

year = "2011",

month = jun,

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doi = "10.1016/j.bmcl.2011.04.137",

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AU - Ying, Lai Qiang

AU - Branchaud, Bruce P.

PY - 2011/6/15

Y1 - 2011/6/15

N2 - New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.

AB - New BODIPY-based pH probes have been designed with excitation and emission wavelengths suitable for fluorescence microscopy and flow cytometry. These pH probes are cell-permeable, selectively label lysosomes, and can be used for noninvasive monitoring of lysosomal pH changes during physiological and pathological processes.

KW - Cell imaging

KW - Confocal microscopy

KW - Fluorescent probe

KW - Lysosome

KW - pH sensor

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 12

ER -

Selective labeling and monitoring pH changes of lysosomes in living cells with fluorogenic pH sensors

Abstract

Keywords

ASJC Scopus subject areas

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