Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex

Yuichi Kamikubo, G. Loredana Mendolicchio, Antonella Zampolli, Patrizia Marchese, Andrea S. Rothmeier, Jennifer Nagrampa Orje, Andrew J. Gale, Sriram Krishnaswamy, Andras Gruber, Henrik Østergaard, Lars C. Petersen, Wolfram Ruf, Zaverio M. Ruggeri

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Safe and effective antithrombotic therapy requires understanding of mechanisms that contribute to pathological thrombosis but have a lesser impact on hemostasis. We found that the extrinsic tissue factor (TF) coagulation initiation complex can selectively activate the antihemophilic cofactor, FVIII, triggering the hemostatic intrinsic coagulation pathway independently of thrombin feedback loops. In a mouse model with a relatively mild thrombogenic lesion, TF-dependent FVIII activation sets the threshold for thrombus formation through contact phase-generated FIXa. In vitro, FXa stably associated with TF-FVIIa activates FVIII, but not FV. Moreover, nascent FXa product of TF-FVIIa can transiently escape the slow kinetics of Kunitz-type inhibition by TF pathway inhibitor and preferentially activates FVIII over FV. Thus, TF synergistically primes FIXa-dependent thrombin generation independently of cofactor activation by thrombin. Accordingly, FVIIa mutants deficient in direct TF-dependent thrombin generation, but preserving FVIIIa generation by nascent FXa, can support intrinsic pathway coagulation. In ex vivo flowing blood, a TF-FVIIa mutant complex with impaired free FXa generation but activating both FVIII and FIX supports efficient FVIII-dependent thrombus formation. Thus, a previously unrecognized TF-initiated pathway directly yielding FVIIIa-FIXa intrinsic tenase complex may be prohemostatic before further coagulation amplification by thrombin-dependent feedback loops enhances the risk of thrombosis.

Original languageEnglish (US)
Pages (from-to)1661-1670
Number of pages10
JournalBlood
Volume130
Issue number14
DOIs
StatePublished - Oct 5 2017

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Factor VIII
Thromboplastin
Chemical activation
Tissue
Thrombin
Coagulation
Thrombosis
cancer procoagulant
Feedback
Hemostatics
Hemostasis
Amplification
Blood
Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Kamikubo, Y., Mendolicchio, G. L., Zampolli, A., Marchese, P., Rothmeier, A. S., Orje, J. N., ... Ruggeri, Z. M. (2017). Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex. Blood, 130(14), 1661-1670. https://doi.org/10.1182/blood-2017-02-767079

Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex. / Kamikubo, Yuichi; Mendolicchio, G. Loredana; Zampolli, Antonella; Marchese, Patrizia; Rothmeier, Andrea S.; Orje, Jennifer Nagrampa; Gale, Andrew J.; Krishnaswamy, Sriram; Gruber, Andras; Østergaard, Henrik; Petersen, Lars C.; Ruf, Wolfram; Ruggeri, Zaverio M.

In: Blood, Vol. 130, No. 14, 05.10.2017, p. 1661-1670.

Research output: Contribution to journalArticle

Kamikubo, Y, Mendolicchio, GL, Zampolli, A, Marchese, P, Rothmeier, AS, Orje, JN, Gale, AJ, Krishnaswamy, S, Gruber, A, Østergaard, H, Petersen, LC, Ruf, W & Ruggeri, ZM 2017, 'Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex', Blood, vol. 130, no. 14, pp. 1661-1670. https://doi.org/10.1182/blood-2017-02-767079
Kamikubo Y, Mendolicchio GL, Zampolli A, Marchese P, Rothmeier AS, Orje JN et al. Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex. Blood. 2017 Oct 5;130(14):1661-1670. https://doi.org/10.1182/blood-2017-02-767079
Kamikubo, Yuichi ; Mendolicchio, G. Loredana ; Zampolli, Antonella ; Marchese, Patrizia ; Rothmeier, Andrea S. ; Orje, Jennifer Nagrampa ; Gale, Andrew J. ; Krishnaswamy, Sriram ; Gruber, Andras ; Østergaard, Henrik ; Petersen, Lars C. ; Ruf, Wolfram ; Ruggeri, Zaverio M. / Selective factor VIII activation by the tissue factor–factor VIIa–factor Xa complex. In: Blood. 2017 ; Vol. 130, No. 14. pp. 1661-1670.
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