Selective control of SNARE recycling by golgi retention

Masayoshi Fukasawa, Anda Cornea, Oleg Varlamov

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    Two distinct sets of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) catalyze membrane fusion in the cis-Golgi and trans-Golgi. The mechanism that controls Golgi localization of SNAREs remains largely unknown. Here we tested three potential mechanisms, including vesicle recycling between the Golgi and the endoplasmic reticulum, partitioning in Golgi lipid microdomains, and selective intra-Golgi retention. Recycling rates showed a linear relationship with intra-Golgi mobility of SNAREs. The cis-Golgi SNAREs had higher mobility than intra-Golgi SNAREs, whereas vesicle SNAREs had higher mobility than target membrane SNAREs. The differences in SNARE mobility were not due to preferential partitioning into detergent-resistant membrane microdomains. We propose that intra-Golgi retention precludes entropy-driven redistribution of SNAREs to the endoplasmic reticulum and endocytic compartments.

    Original languageEnglish (US)
    Pages (from-to)2377-2384
    Number of pages8
    JournalFEBS Letters
    Volume587
    Issue number15
    DOIs
    StatePublished - Aug 2 2013

    Fingerprint

    SNARE Proteins
    Recycling
    Membranes
    Endoplasmic Reticulum
    Membrane Microdomains
    Membrane Fusion
    Entropy
    Detergents
    Fusion reactions
    Lipids

    Keywords

    • Dynamic equilibrium
    • Endoplasmic reticulum
    • Entropy
    • ER
    • Golgi
    • Golgi tether
    • i-SNARE Homotypic fusion
    • Lipid raft Membrane fusion
    • Recycling
    • Retention
    • SNARE
    • Vesicle

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Genetics
    • Molecular Biology
    • Structural Biology

    Cite this

    Selective control of SNARE recycling by golgi retention. / Fukasawa, Masayoshi; Cornea, Anda; Varlamov, Oleg.

    In: FEBS Letters, Vol. 587, No. 15, 02.08.2013, p. 2377-2384.

    Research output: Contribution to journalArticle

    Fukasawa, Masayoshi ; Cornea, Anda ; Varlamov, Oleg. / Selective control of SNARE recycling by golgi retention. In: FEBS Letters. 2013 ; Vol. 587, No. 15. pp. 2377-2384.
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