Selective control of SNARE recycling by golgi retention

Masayoshi Fukasawa, Anda Cornea, Oleg Varlamov

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Two distinct sets of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) catalyze membrane fusion in the cis-Golgi and trans-Golgi. The mechanism that controls Golgi localization of SNAREs remains largely unknown. Here we tested three potential mechanisms, including vesicle recycling between the Golgi and the endoplasmic reticulum, partitioning in Golgi lipid microdomains, and selective intra-Golgi retention. Recycling rates showed a linear relationship with intra-Golgi mobility of SNAREs. The cis-Golgi SNAREs had higher mobility than intra-Golgi SNAREs, whereas vesicle SNAREs had higher mobility than target membrane SNAREs. The differences in SNARE mobility were not due to preferential partitioning into detergent-resistant membrane microdomains. We propose that intra-Golgi retention precludes entropy-driven redistribution of SNAREs to the endoplasmic reticulum and endocytic compartments.

Original languageEnglish (US)
Pages (from-to)2377-2384
Number of pages8
JournalFEBS Letters
Volume587
Issue number15
DOIs
StatePublished - Aug 2 2013
Externally publishedYes

Keywords

  • Dynamic equilibrium
  • ER
  • Endoplasmic reticulum
  • Entropy
  • Golgi
  • Golgi tether
  • Lipid raft Membrane fusion
  • Recycling
  • Retention
  • SNARE
  • Vesicle
  • i-SNARE Homotypic fusion

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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