Selective amplification of simian immunodeficiency virus genotypes after intrarectal inoculation of rhesus monkeys

Parul Trivedi, Krista K. Meyer, Daniel Streblow, Brenda L. Preuninger, Kevin T. Schultz, C. David Pauza

Research output: Contribution to journalArticle

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Abstract

Animal models for sexual transmission of human immunodeficiency virus can define the influences of virus type, dose, and route of inoculation on infection and clinical outcome. We used an uncloned simian immunodeficiency virus stock (SIVmac) to inoculate cells in vitro and to inoculate rhesus monkeys by intravenous and intrarectal routes. The distribution of virus genotypes present in each of these infection examples was characterized by DNA sequence analysis of viral long terminal repeats (LTRs). Our analysis of LTR sequences from in vitro and in vivo infections revealed three main genotypes: one genotype was observed only for in vitro infection, and two other genotypes were recovered only from infected animals. By comparing animals inoculated with high intrarectal doses of SIVmac and those inoculated with low doses, we demonstrated that unique subsets of the stock were selected after intrarectal infection. Our findings indicate that minor genotypes present in the stock cross the rectal mucosa and are amplified selectively to become prominent in peripheral blood mononuclear cells from acutely infected animals. Studies with a molecular recombinant of SIV and human immunodeficiency virus type 1 sequences, SHIV, showed that viral LTR sequences do not undergo especially rapid sequence variation or rearrangement after intrarectal inoculation. The mucosal barrier exerts a significant influence on infection and disease progression by reducing the efficiency of SIVmac infection and by permitting distinct, pathogenic genotypes to become established in the host.

Original languageEnglish (US)
Pages (from-to)7649-7653
Number of pages5
JournalJournal of Virology
Volume68
Issue number11
StatePublished - Nov 1994
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
Macaca mulatta
terminal repeat sequences
Terminal Repeat Sequences
Genotype
genotype
Infection
infection
dosage
Viruses
viruses
animals
Human immunodeficiency virus
mononuclear leukocytes
Human immunodeficiency virus 1
DNA Sequence Analysis
disease course
mucosa
Disease Progression

ASJC Scopus subject areas

  • Immunology

Cite this

Trivedi, P., Meyer, K. K., Streblow, D., Preuninger, B. L., Schultz, K. T., & Pauza, C. D. (1994). Selective amplification of simian immunodeficiency virus genotypes after intrarectal inoculation of rhesus monkeys. Journal of Virology, 68(11), 7649-7653.

Selective amplification of simian immunodeficiency virus genotypes after intrarectal inoculation of rhesus monkeys. / Trivedi, Parul; Meyer, Krista K.; Streblow, Daniel; Preuninger, Brenda L.; Schultz, Kevin T.; Pauza, C. David.

In: Journal of Virology, Vol. 68, No. 11, 11.1994, p. 7649-7653.

Research output: Contribution to journalArticle

Trivedi, P, Meyer, KK, Streblow, D, Preuninger, BL, Schultz, KT & Pauza, CD 1994, 'Selective amplification of simian immunodeficiency virus genotypes after intrarectal inoculation of rhesus monkeys', Journal of Virology, vol. 68, no. 11, pp. 7649-7653.
Trivedi, Parul ; Meyer, Krista K. ; Streblow, Daniel ; Preuninger, Brenda L. ; Schultz, Kevin T. ; Pauza, C. David. / Selective amplification of simian immunodeficiency virus genotypes after intrarectal inoculation of rhesus monkeys. In: Journal of Virology. 1994 ; Vol. 68, No. 11. pp. 7649-7653.
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