Selective activation of androgen receptors in the subcortical brain of male cynomolgus macaques by physiological hormone levels and its relationship to androgen-dependent aromatase activity

John A. Resko, Peter B. Connolly, Charles Roselli, Salah E. Abdelgadir, Jerome V A Choate

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17 Citations (Scopus)

Abstract

Aromatase activity (AA) is androgen dependent and independent in subcortical regions of the nonhuman primate brain, but the correlation of androgen receptor (AR) content with AA has not been demonstrated. Thus, we castrated 10 adult male cynomolgus monkeys (Macaca fasicularis) and divided them into 2 groups. One group (n = 6) received empty Silastic capsules, whereas the second group (n = 4) received Silastic capsules filled with testosterone (T). Animals were killed after 3 weeks. Microsomal AA and cytosolic and nuclear AR were determined in specific brain regions dissected from frozen sections. Sera from T-treated subjects contained T, dihydrotestosterone, and LH levels that were not significantly different from the precastration amounts (P <0.05). Cytosolic AR concentrations declined after T treatment in 12 of 20 brain areas studied (P <0.05). Nuclear AR levels, on the other hand, were significantly elevated after T treatment (activated) only in the ventral medial nucleus (VMN) and infundibular nucleus/median eminence (P <0.05). AA distribution was significantly different (P <0.05) among 20 brain nuclei and subregions. The highest activities were found in the bed nucleus of the stria terminalis, the medial preoptic area, the medial and cortical amygdala, and the VMN. Lesser activities were found in other brain regions. Physiological concentrations of T increased AA only in the VMN and infundibular nucleus-median eminence (P <0.05). These data suggest that physiological levels of androgens are effective in regulating AA only in those brain areas in which AR are activated.

Original languageEnglish (US)
Pages (from-to)1588-1593
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Volume76
Issue number6
StatePublished - Jun 1993

Fingerprint

Aromatase
Androgen Receptors
Macaca
Androgens
Brain
Chemical activation
Hormones
Median Eminence
Arcuate Nucleus of Hypothalamus
Cytoplasmic and Nuclear Receptors
Capsules
Septal Nuclei
Preoptic Area
Macaca fascicularis
Dihydrotestosterone
Frozen Sections
Primates
Testosterone
Animals
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Selective activation of androgen receptors in the subcortical brain of male cynomolgus macaques by physiological hormone levels and its relationship to androgen-dependent aromatase activity. / Resko, John A.; Connolly, Peter B.; Roselli, Charles; Abdelgadir, Salah E.; Choate, Jerome V A.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 76, No. 6, 06.1993, p. 1588-1593.

Research output: Contribution to journalArticle

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