Selection of encephalitogenic rat T-lymphocyte clones recognizing an immunodominant epitope on myelin basic protein

Y. K. Chou, Arthur Vandenbark, R. E. Jones, G. Hashim, Halina Offner

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Abstract

Using the soft agar-cloning technique, we isolated 13 T-cell clones from guinea pig basic protein (GP-BP)-specific T-cell lines derived from Lewis rats. The clonal frequency was approximately 2.5 x 10-5. Each of these clones had a similar but not identical pattern of response to a battery of synthetic peptides representing overlapping epitopes in the encephalitogenic region for Lewis rats (68-69 sequence). All clones responded to the minimal encephalitogenic sequence (residues 72-84) restricted by I-A but not I-E molecules, and all transferred clinical experimental autoimmune encephalomyelitis (EAE) and delayed-type hypersensitivity (DTH) reaction to naive rats. Phenotypically, the clones were W3/13+ (total T), W3/25+ (T helper), and OX-22+ (DTH associated). This report demonstrates for the first time the applicability of the soft agar-cloning technique for obtaining encephalitogenic T-cell clones. The exclusive recovery of 72-84-specific T-cell clones after only two rounds of stimulation with GP-BP indicates the immunodominance of this epitope and the power of the line selection technique for obtaining encephalitogenic T-cell specificities.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalJournal of Neuroscience Research
Volume22
Issue number2
StatePublished - 1989

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Immunodominant Epitopes
Myelin Basic Protein
Clone Cells
T-Lymphocytes
Delayed Hypersensitivity
Agar
Organism Cloning
Epitopes
Guinea Pigs
T-Cell Antigen Receptor Specificity
Autoimmune Experimental Encephalomyelitis
Proteins
Cell Line
Peptides

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Selection of encephalitogenic rat T-lymphocyte clones recognizing an immunodominant epitope on myelin basic protein. / Chou, Y. K.; Vandenbark, Arthur; Jones, R. E.; Hashim, G.; Offner, Halina.

In: Journal of Neuroscience Research, Vol. 22, No. 2, 1989, p. 181-187.

Research output: Contribution to journalArticle

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