Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples

C. Ortiz de Solorzano, A. Sarti, E. Garcia Rodriguez, A. Jones, D. Sudar, D. Pinkel, Joe Gray, R. Malladi, S. J. Lockett

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a segmentation method for cell nuclei in 3D confocal images of tissue specimens, and discuss some new approaches that could substantially improve its performance. The current method combines automatic segmentation algorithms with graphic visualization and interactive classification to achieve fast and accurate segmentation. However, the edge fidelity is low for some nuclei and other nuclei are so tightly clustered that they can not be segmented. An approach based on curvature-based flow is proposed for improving edge fidelity and an approach based on the Hough transform is proposed for segmenting clusters of nuclei.

Original languageEnglish (US)
Title of host publicationProceedings of the Joint Conference on Information Sciences
EditorsK. Li, Y. Pan, P.P. Wang, A. Al-Dhelaan, H. Da Cheng, Y. Pan, P.P. Wang, K. Li, A. Al-Dhelaan, H. Da Cheng
Pages187-190
Number of pages4
Volume4
StatePublished - 1998
Externally publishedYes
Event4th International Conference on Computer Science and Informatics, JCIS 1998, 1st International Workshop on High Performance, 1st International Workshop on Computer Vision, Pattern Recognition and Image Processing Volume 4 - Research Triangle Park, NC, United States
Duration: Oct 23 1998Oct 28 1998

Other

Other4th International Conference on Computer Science and Informatics, JCIS 1998, 1st International Workshop on High Performance, 1st International Workshop on Computer Vision, Pattern Recognition and Image Processing Volume 4
CountryUnited States
CityResearch Triangle Park, NC
Period10/23/9810/28/98

Fingerprint

Hough transforms
Microscopes
Visualization
Cells
Tissue

ASJC Scopus subject areas

  • Computer Science(all)

Cite this

Ortiz de Solorzano, C., Sarti, A., Rodriguez, E. G., Jones, A., Sudar, D., Pinkel, D., ... Lockett, S. J. (1998). Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples. In K. Li, Y. Pan, P. P. Wang, A. Al-Dhelaan, H. Da Cheng, Y. Pan, P. P. Wang, K. Li, A. Al-Dhelaan, ... H. Da Cheng (Eds.), Proceedings of the Joint Conference on Information Sciences (Vol. 4, pp. 187-190)

Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples. / Ortiz de Solorzano, C.; Sarti, A.; Rodriguez, E. Garcia; Jones, A.; Sudar, D.; Pinkel, D.; Gray, Joe; Malladi, R.; Lockett, S. J.

Proceedings of the Joint Conference on Information Sciences. ed. / K. Li; Y. Pan; P.P. Wang; A. Al-Dhelaan; H. Da Cheng; Y. Pan; P.P. Wang; K. Li; A. Al-Dhelaan; H. Da Cheng. Vol. 4 1998. p. 187-190.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ortiz de Solorzano, C, Sarti, A, Rodriguez, EG, Jones, A, Sudar, D, Pinkel, D, Gray, J, Malladi, R & Lockett, SJ 1998, Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples. in K Li, Y Pan, PP Wang, A Al-Dhelaan, H Da Cheng, Y Pan, PP Wang, K Li, A Al-Dhelaan & H Da Cheng (eds), Proceedings of the Joint Conference on Information Sciences. vol. 4, pp. 187-190, 4th International Conference on Computer Science and Informatics, JCIS 1998, 1st International Workshop on High Performance, 1st International Workshop on Computer Vision, Pattern Recognition and Image Processing Volume 4, Research Triangle Park, NC, United States, 10/23/98.
Ortiz de Solorzano C, Sarti A, Rodriguez EG, Jones A, Sudar D, Pinkel D et al. Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples. In Li K, Pan Y, Wang PP, Al-Dhelaan A, Da Cheng H, Pan Y, Wang PP, Li K, Al-Dhelaan A, Da Cheng H, editors, Proceedings of the Joint Conference on Information Sciences. Vol. 4. 1998. p. 187-190
Ortiz de Solorzano, C. ; Sarti, A. ; Rodriguez, E. Garcia ; Jones, A. ; Sudar, D. ; Pinkel, D. ; Gray, Joe ; Malladi, R. ; Lockett, S. J. / Segmentation of Cell Nuclei from Confocal Microscope Images of Thick Tissue Samples. Proceedings of the Joint Conference on Information Sciences. editor / K. Li ; Y. Pan ; P.P. Wang ; A. Al-Dhelaan ; H. Da Cheng ; Y. Pan ; P.P. Wang ; K. Li ; A. Al-Dhelaan ; H. Da Cheng. Vol. 4 1998. pp. 187-190
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