Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway

David Lewinsohn, Jeff E. Grotzke, Amy S. Heinzel, LiQing Zhu, Pamela J. Ovendale, Mark Johnson, Mark R. Alderson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

CD8+ T cells play an important role in the host response to infection with Mycobacterium tuberculosis (Mtb). Mtb resides in an arrested phagosome that is phenotypically similar to an early endosome. The mechanisms by which Mtb-derived Ags gain access to the HLA-I-processing pathway are incompletely characterized. Studies with CD8+ T cell lines have suggested that Mtb Ags gain access to the HLA-I pathway in an alternate vacuolar pathway that is both brefeldin A (BFA) and TAP independent. To define the requirements of entry of Ag into the HLA-I pathway, we have used human CD8 + T cell clones specific for the secreted Mtb Ag CFP10. Human monocyte-derived dendritic cells were pulsed with CFP10 expressed in a recombinant adenovirus, surface adsorbed to microspheres, or in its native form by Mtb. When delivered by adenovirus, processing and presentation of CFP10 were blocked by both BFA and the proteasomal blocker lactacystin. In contrast, processing of CFP10 adsorbed to the surface of microspheres was not affected by either of these Ag-processing inhibitors. BFA, lactacystin, and TAP inhibition blocked the recognition of Mtb-infected dendritic cells, suggesting that processing was via a cytosolic pathway for this secreted protein Ag. We conclude that secreted proteins from Mtb can be processed in a BFA- and proteasome-dependent manner, consistent with egress of Ag into the cytosol and subsequent loading of proteasomally derived peptides.

Original languageEnglish (US)
Pages (from-to)437-442
Number of pages6
JournalJournal of Immunology
Volume177
Issue number1
StatePublished - Jul 1 2006

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Histocompatibility Antigens Class I
Antigen Presentation
Mycobacterium tuberculosis
Brefeldin A
Proteins
T-Lymphocytes
Microspheres
Adenoviridae
Dendritic Cells
Phagosomes
Endosomes
Proteasome Endopeptidase Complex
Cytosol
Monocytes
Clone Cells
Cell Line
Peptides
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Lewinsohn, D., Grotzke, J. E., Heinzel, A. S., Zhu, L., Ovendale, P. J., Johnson, M., & Alderson, M. R. (2006). Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway. Journal of Immunology, 177(1), 437-442.

Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway. / Lewinsohn, David; Grotzke, Jeff E.; Heinzel, Amy S.; Zhu, LiQing; Ovendale, Pamela J.; Johnson, Mark; Alderson, Mark R.

In: Journal of Immunology, Vol. 177, No. 1, 01.07.2006, p. 437-442.

Research output: Contribution to journalArticle

Lewinsohn, D, Grotzke, JE, Heinzel, AS, Zhu, L, Ovendale, PJ, Johnson, M & Alderson, MR 2006, 'Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway', Journal of Immunology, vol. 177, no. 1, pp. 437-442.
Lewinsohn, David ; Grotzke, Jeff E. ; Heinzel, Amy S. ; Zhu, LiQing ; Ovendale, Pamela J. ; Johnson, Mark ; Alderson, Mark R. / Secreted proteins from Mycobacterium tuberculosis gain access to the cytosolic MHC class-I antigen-processing pathway. In: Journal of Immunology. 2006 ; Vol. 177, No. 1. pp. 437-442.
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