Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells

Jinyoung Kim, David Erikson, Robert C. Burghardt, Thomas E. Spencer, Guoyao Wu, Kayla J. Bayless, Greg A. Johnson, Fuller W. Bazer

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Attachment and migration of trophectoderm (Tr) cells, hallmarks of blastocyst implantation in mammals, are unique uterine events. Secreted phosphoprotein 1 (SPP1) in the uterus binds integrins on conceptus Tr and uterine luminal epithelium (LE), affecting cell-cell and cell-matrix interactions. The signal transduction pathways activated by SPP1 and integrins in conceptuses have not been elucidated. Results of this study demonstrate that SPP1 binds αvβ3 and α5β1 integrins to induce focal adhesion assembly, a prerequisite for adhesion and migration of Tr, through activation of: 1) P70S6K via crosstalk between FRAP1/mTOR and MAPK pathways; 2) mTOR, PI3K, MAPK3/MAPK1 (Erk1/2) and MAPK14 (p38) signaling to stimulate Tr cell migration; and 3) focal adhesion assembly and myosin II motor activity to induce migration of Tr cells. These cell signaling pathways, acting in concert, mediate adhesion, migration and cytoskeletal remodeling of Tr cells essential for expansion and elongation of conceptuses and attachment to uterine LE for implantation.

Original languageEnglish (US)
Pages (from-to)369-382
Number of pages14
JournalMatrix Biology
Volume29
Issue number5
DOIs
StatePublished - Jun 2010
Externally publishedYes

Fingerprint

Osteopontin
Integrins
Cell Movement
Focal Adhesions
Mitogen-Activated Protein Kinase 14
Epithelium
Myosin Type II
70-kDa Ribosomal Protein S6 Kinases
Phosphatidylinositol 3-Kinases
Cell Communication
Uterus
Mammals
Signal Transduction
Motor Activity

Keywords

  • Implantation
  • Integrins
  • Mitogen activated protein kinases
  • MTOR
  • Secreted phosphoprotein 1
  • Trophectoderm

ASJC Scopus subject areas

  • Molecular Biology
  • Medicine(all)

Cite this

Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells. / Kim, Jinyoung; Erikson, David; Burghardt, Robert C.; Spencer, Thomas E.; Wu, Guoyao; Bayless, Kayla J.; Johnson, Greg A.; Bazer, Fuller W.

In: Matrix Biology, Vol. 29, No. 5, 06.2010, p. 369-382.

Research output: Contribution to journalArticle

Kim, Jinyoung ; Erikson, David ; Burghardt, Robert C. ; Spencer, Thomas E. ; Wu, Guoyao ; Bayless, Kayla J. ; Johnson, Greg A. ; Bazer, Fuller W. / Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells. In: Matrix Biology. 2010 ; Vol. 29, No. 5. pp. 369-382.
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