Secreted meningeal chemokines, but not VEGFA, modulate the migratory properties of medulloblastoma cells

Monika A. Davare, Sangeet Lal, Jennifer L. Peckham, Suresh I. Prajapati, Sakir H. Gultekin, Brian P. Rubin, Charles Keller

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Leptomeningeal metastasis is a cause of morbidity and mortality in medulloblastoma, but the understanding of molecular mechanisms driving this process is nascent. In this study, we examined the secretory chemokine profile of medulloblastoma cells (DAOY) and a meningothelial cell line (BMEN1). Conditioned media (CM) of meningothelial cells increased adhesion, spreading and migration of medulloblastoma. VEGFA was identified at elevated levels in the CM from BMEN1 cells (as compared to DAOY CM); however, recombinant VEGFA alone was insufficient to enhance medulloblastoma cell migration. In addition, bevacizumab, the VEGFA scavenging monoclonal antibody, did not block the migratory phenotype induced by the CM. These results reveal that paracrine factors secreted by meningothelial cells can influence migration and adherence of medulloblastoma tumor cells, but VEGFA may not be a specific target for therapeutic intervention in this context.

Original languageEnglish (US)
Pages (from-to)555-560
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume450
Issue number1
DOIs
StatePublished - Jul 18 2014

Keywords

  • Leptomeningeal metastasis
  • Medulloblastoma
  • VEGFA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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