Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure

Janna Merte, Devon Jensen, Kevin Wright, Sarah Sarsfield, Yanshu Wang, Randy Schekman, David D. Ginty

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Craniorachischisis is a rare but severe birth defect that results in a completely open neural tube. Mouse mutants in planar cell polarity (PCP) signalling components have deficits in the morphological movements of convergent extension that result in craniorachischisis. Using a forward genetic screen in mice, we identified Sec24b, a cargo-sorting member of the core complex of the endoplasmic reticulum (ER)-to-Golgi transport vesicle COPII, as critical for neural tube closure. Sec24b Y613 mutant mice exhibit craniorachischisis, deficiencies in convergent extension and other PCP-related phenotypes. Vangl2, a key component of the PCP-signalling pathway critical for convergent extension, is selectively sorted into COPII vesicles by Sec24b. Moreover, Sec24b Y613 genetically interacts with a loss-of-function Vangl2 allele (Vangl2 LP), causing a marked increase in the prevalence of spina bifida. Interestingly, the Vangl2 looptail point mutants Vangl2 D255E and Vangl2 S464N, known to cause defects in convergent extension, fail to sort into COPII vesicles and are trapped in the ER. Thus, during COPII vesicle formation, Sec24b shows cargo specificity for a core PCP component, Vangl2, of which proper ER-to-Golgi transport is essential for the establishment of PCP, convergent extension and closure of the neural tube.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalNature Cell Biology
Volume12
Issue number1
DOIs
StatePublished - Jan 2010
Externally publishedYes

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Cell Polarity
Neural Tube
Neural Tube Defects
Endoplasmic Reticulum
Transport Vesicles
Spinal Dysraphism
Critical Pathways
Cellular Structures
Alleles
Phenotype

ASJC Scopus subject areas

  • Cell Biology

Cite this

Merte, J., Jensen, D., Wright, K., Sarsfield, S., Wang, Y., Schekman, R., & Ginty, D. D. (2010). Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure. Nature Cell Biology, 12(1), 41-46. https://doi.org/10.1038/ncb2002

Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure. / Merte, Janna; Jensen, Devon; Wright, Kevin; Sarsfield, Sarah; Wang, Yanshu; Schekman, Randy; Ginty, David D.

In: Nature Cell Biology, Vol. 12, No. 1, 01.2010, p. 41-46.

Research output: Contribution to journalArticle

Merte, J, Jensen, D, Wright, K, Sarsfield, S, Wang, Y, Schekman, R & Ginty, DD 2010, 'Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure', Nature Cell Biology, vol. 12, no. 1, pp. 41-46. https://doi.org/10.1038/ncb2002
Merte, Janna ; Jensen, Devon ; Wright, Kevin ; Sarsfield, Sarah ; Wang, Yanshu ; Schekman, Randy ; Ginty, David D. / Sec24b selectively sorts Vangl2 to regulate planar cell polarity during neural tube closure. In: Nature Cell Biology. 2010 ; Vol. 12, No. 1. pp. 41-46.
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